Production in Trichoderma reesei of three xylanases from Chaetomium thermophilum: A recombinant thermoxylanase for biobleaching of kraft pulp

Arja Mäntylä (Corresponding Author), Marja Paloheimo, Satu Hakola, Emilia: Leskinen, Sanna Lindberg, Jarno Kallio, Jari Vehmaanperä, Raija Lantto, Pirkko Suominen

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Abstract

Abstract Three endoxylanase genes were cloned from the thermophilic fungus Chaetomium thermophilum CBS 730.95. All genes contained the typical consensus sequence of family 11 glycoside hydrolases. Genomic copies of Ct xyn11A, Ct xyn11B, and Ct xyn11C were expressed in the filamentous fungus T. reesei under the control of the strong T. reesei cel7A (cellobiohydrolase 1, cbh1) promoter. The molecular masses of the Ct Xyn11A, Ct Xyn11B, and Ct Xyn11C proteins on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were 27, 23, and 22 kDa, respectively. Ct Xyn11A was produced almost as efficiently as the homologous xylanase II from a corresponding single-copy transformant strain. Ct Xyn11B production level was approximately half of that of Ct Xyn11A. The amount of Ct Xyn11C was remarkably lower. Ct Xyn11A had the highest temperature optimum and stability of the recombinant xylanases and the highest activity at acid-neutral pH (pH 5–7). It was the most suitable for industrial bleaching of kraft pulp at high temperature.
Original languageEnglish
Pages (from-to)377 - 386
JournalApplied Microbiology and Biotechnology
Volume76
Issue number2
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Keywords

  • Trichoderma reesei
  • xylanases
  • Chaetomium thermophilum
  • recombinant proteins
  • biobleaching
  • bleaching
  • pulp
  • Kraft pulp

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