Production of biologically active recombinant avidin in baculovirus-infected insect cells

K. Airenne, Christian Oker-Blom, V. Marjomäki, E. Bayer, M. Wilchek, M. Kulomaa

Research output: Contribution to journalArticleScientificpeer-review

51 Citations (Scopus)

Abstract

An efficient lepidopteran insect cell system was established for the expression of a recombinant form of chicken egg-white avidin. The gene product was obtained in both secreted and intracellular forms, and biologically active recombinant avidin was isolated using affinity chromatography on an iminobiotin–agarose column. Similar to the known quaternary structure of the native egg-white protein, the purified recombinant protein was glycosylated and assembled mainly into tetramers. Like native avidin, the recombinant tetramer also exhibited a high level of thermostability, and was further stabilized upon binding biotin. The biotin-binding and structural properties of the recombinant avidin are thus similar to those of the natural egg-white protein, and the insect system is appropriate both for future site-directed mutagenesis studies and for the production of avidin fusion proteins.

Original languageEnglish
Pages (from-to)100 - 108
Number of pages9
JournalProtein Expression and Purification
Volume9
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Avidin
Baculoviridae
Insects
Egg Proteins
Biotin
Egg White
Site-Directed Mutagenesis
Affinity Chromatography
Recombinant Proteins
Chickens
Genes
Proteins

Cite this

Airenne, K., Oker-Blom, C., Marjomäki, V., Bayer, E., Wilchek, M., & Kulomaa, M. (1997). Production of biologically active recombinant avidin in baculovirus-infected insect cells. Protein Expression and Purification, 9(1), 100 - 108. https://doi.org/10.1006/prep.1996.0660
Airenne, K. ; Oker-Blom, Christian ; Marjomäki, V. ; Bayer, E. ; Wilchek, M. ; Kulomaa, M. / Production of biologically active recombinant avidin in baculovirus-infected insect cells. In: Protein Expression and Purification. 1997 ; Vol. 9, No. 1. pp. 100 - 108.
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Airenne, K, Oker-Blom, C, Marjomäki, V, Bayer, E, Wilchek, M & Kulomaa, M 1997, 'Production of biologically active recombinant avidin in baculovirus-infected insect cells', Protein Expression and Purification, vol. 9, no. 1, pp. 100 - 108. https://doi.org/10.1006/prep.1996.0660

Production of biologically active recombinant avidin in baculovirus-infected insect cells. / Airenne, K.; Oker-Blom, Christian; Marjomäki, V.; Bayer, E.; Wilchek, M.; Kulomaa, M.

In: Protein Expression and Purification, Vol. 9, No. 1, 1997, p. 100 - 108.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Airenne, K.

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AU - Kulomaa, M.

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