Abstract
Increased interest in marine resources has led to
increased screening of marine fungi for novel bioactive
compounds and considerable effort is being invested in
discovering these metabolites. For compound discovery,
small-scale cultures are adequate, but agitated
bioreactors are desirable for larger-scale production.
Calcarisporium sp. KF525 has recently been described to
produce calcaride A, a cyclic polyester with antibiotic
activity, in agitated flasks. Here, we describe
improvements in the production of calcaride A in both
flasks (13-fold improvement) and stirred bioreactors
(200-fold improvement). Production of calcaride A in
bioreactors was initially substantially lower than in
shaken flasks. The cultivation pH (reduced from 6.8 to
Original language | English |
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Pages (from-to) | 3992-4005 |
Journal | Marine Drugs |
Volume | 13 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2015 |
MoE publication type | A1 Journal article-refereed |
Keywords
- marine fungi
- Calcarisporium
- calcaride A
- stirred tank bioreactor
- pH
- macrocylic polyester