Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway

Peter Richard, Ritva Verho, Mikko Putkonen, John Londesborough, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

95 Citations (Scopus)

Abstract

The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalFEMS Yeast Research
Volume3
Issue number2
DOIs
Publication statusPublished - 1 Apr 2003
MoE publication typeA1 Journal article-refereed

Keywords

  • L-Arabinitol 4-dehydrogenase
  • L-Arabinose
  • L-Xylulose reductase
  • Pentose fermentation
  • Saccharomyces cerevisiae

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