Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway

Peter Richard; Ritva Verho; Mikko Putkonen; John Londesborough; Merja Penttilä

doi:10.1016/S1567-1356(02)00184-8

Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway

Peter Richard^*
, Ritva Verho
, Mikko Putkonen
, John Londesborough
, Merja Penttilä

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

113 Citations (Scopus)

Abstract

The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed.

Original language	English
Pages (from-to)	185-189
Number of pages	5
Journal	FEMS Yeast Research
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/S1567-1356(02)00184-8
Publication status	Published - 1 Apr 2003
MoE publication type	A1 Journal article-refereed

Funding

This work was supported by the ‘Sustainable Use of Natural Resources’ (SUNARE) programme of the Academy of Finland and the research programme ‘VTT Industrial Biotechnology’ (Academy of Finland; Finnish Centre of Excellence programme, 2000–2005, Project no. 64330).

Keywords

L-Arabinitol 4-dehydrogenase
L-Arabinose
L-Xylulose reductase
Pentose fermentation
Saccharomyces cerevisiae

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10.1016/S1567-1356(02)00184-8

Cite this

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title = "Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway",

abstract = "The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed.",

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AU - Richard, Peter

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AU - Londesborough, John

AU - Penttilä, Merja

PY - 2003/4/1

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AB - The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed.

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Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway

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