Production of functional IgM Fab fragments by Saccharomyces cerevisiae

J. Edqvist, Sirkka Keränen, Merja Penttilä, K. B. Strroaby, Jonathan K.C. Knowles

    Research output: Contribution to journalArticleScientificpeer-review

    12 Citations (Scopus)

    Abstract

    The aim of this study was to express and secrete functional mouse IgM fragments in yeast. The heavy chain cDNA was truncated at two different sites, yielding genes coding for the complete VH domain. In one of the truncated genes, the CH1 domain is complete, while in the other gene 18 bp are missing from the 3′ terminus of the CH1 region. Both shortened genes were coexpressed in Saccharomyces cerevisiae with a cDNA gene encoding a full length mouse Ig light chain. We show that only the longer form of the truncated heavy chain together with the light chain produced and secreted functional IgM Fab fragments.

    Original languageEnglish
    Pages (from-to)291-300
    JournalJournal of Biotechnology
    Volume20
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 1991
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Antibody
    • Assembly
    • IgM
    • Immunoglobulin
    • Secretion
    • Yeast

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