Production of functional IgM Fab fragments by Saccharomyces cerevisiae

J. Edqvist; Sirkka Keränen; Merja Penttilä; K. B. Strroaby; Jonathan K.C. Knowles

doi:10.1016/0168-1656(91)90350-5

Production of functional IgM Fab fragments by Saccharomyces cerevisiae

J. Edqvist^*, Sirkka Keränen, Merja Penttilä, K. B. Strroaby, Jonathan K.C. Knowles

^*Corresponding author for this work

Umeå University

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

The aim of this study was to express and secrete functional mouse IgM fragments in yeast. The heavy chain cDNA was truncated at two different sites, yielding genes coding for the complete VH domain. In one of the truncated genes, the CH1 domain is complete, while in the other gene 18 bp are missing from the 3′ terminus of the CH1 region. Both shortened genes were coexpressed in Saccharomyces cerevisiae with a cDNA gene encoding a full length mouse Ig light chain. We show that only the longer form of the truncated heavy chain together with the light chain produced and secreted functional IgM Fab fragments.

Original language	English
Pages (from-to)	291-300
Journal	Journal of Biotechnology
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/0168-1656(91)90350-5
Publication status	Published - 1 Jan 1991
MoE publication type	A1 Journal article-refereed

Keywords

Antibody
Assembly
IgM
Immunoglobulin
Secretion
Yeast

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10.1016/0168-1656(91)90350-5

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title = "Production of functional IgM Fab fragments by Saccharomyces cerevisiae",

abstract = "The aim of this study was to express and secrete functional mouse IgM fragments in yeast. The heavy chain cDNA was truncated at two different sites, yielding genes coding for the complete VH domain. In one of the truncated genes, the CH1 domain is complete, while in the other gene 18 bp are missing from the 3′ terminus of the CH1 region. Both shortened genes were coexpressed in Saccharomyces cerevisiae with a cDNA gene encoding a full length mouse Ig light chain. We show that only the longer form of the truncated heavy chain together with the light chain produced and secreted functional IgM Fab fragments.",

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AU - Edqvist, J.

AU - Keränen, Sirkka

AU - Penttilä, Merja

AU - Strroaby, K. B.

AU - Knowles, Jonathan K.C.

PY - 1991/1/1

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N2 - The aim of this study was to express and secrete functional mouse IgM fragments in yeast. The heavy chain cDNA was truncated at two different sites, yielding genes coding for the complete VH domain. In one of the truncated genes, the CH1 domain is complete, while in the other gene 18 bp are missing from the 3′ terminus of the CH1 region. Both shortened genes were coexpressed in Saccharomyces cerevisiae with a cDNA gene encoding a full length mouse Ig light chain. We show that only the longer form of the truncated heavy chain together with the light chain produced and secreted functional IgM Fab fragments.

AB - The aim of this study was to express and secrete functional mouse IgM fragments in yeast. The heavy chain cDNA was truncated at two different sites, yielding genes coding for the complete VH domain. In one of the truncated genes, the CH1 domain is complete, while in the other gene 18 bp are missing from the 3′ terminus of the CH1 region. Both shortened genes were coexpressed in Saccharomyces cerevisiae with a cDNA gene encoding a full length mouse Ig light chain. We show that only the longer form of the truncated heavy chain together with the light chain produced and secreted functional IgM Fab fragments.

KW - Antibody

KW - Assembly

KW - IgM

KW - Immunoglobulin

KW - Secretion

KW - Yeast

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SN - 0168-1656

VL - 20

SP - 291

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JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 3

ER -

Production of functional IgM Fab fragments by Saccharomyces cerevisiae

Abstract

Keywords

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