Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan

A. Schmidt, Michael Bailey, Anu Vaari, A. Thomsen, Maija Tenkanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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Abstract

In Denmark, the wet-oxidation (WO) process has been used for fractionating wheat straw [10], where most of the hemicellulose is solubilised mainly as oligomers and polymers and the cellulose retained as a solid. In wheat straw, hemicellulose is an arabino-4-O-methylglucurono xylan, which, in addition to xylose, contains arabinose, 4-O-Me-D-glucuronic acid, and acetic acid substituents [9]. Some arabinosyl groups may be esterified with ferulic or p-coumaric acids. Most micro-organisms used for lactate, ethanol or xylitol production do not produce enzymes for hemicellulose hydrolysis. Acid or enzymatic hydrolysis is needed to convert the sugar polymers to monomers. A problem associated with dilute acid hydrolysis is the poor fermentability of the produced hydrolysates compared with enzymatic hydrolysis. This study investigated the utilisation of wet-oxidised wheat straw xylan (WO-xylan) for production of enzymes for hydrolysis. Three fungal strains (Aspergillus oryzae, Aspergillus fumigatus, and Trichoderma reesei) were cultivated on WO-xylan using xylan (Lenzig AG) as a supplementary carbon-source. A. oryzae was selected for production of enzymes in larger scale.
Original languageEnglish
Title of host publication2nd European Symposium on Enzymes in Grain Processing, ESEPG-2
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages267-271
ISBN (Electronic)951-38-5707-7
ISBN (Print)951-38-5706-9
Publication statusPublished - 2000
MoE publication typeA4 Article in a conference publication
EventEuropean Symposium on Enzymes in Grain Processing, ESEGP-2 - Helsinki, Finland
Duration: 8 Dec 199910 Dec 1999

Publication series

SeriesVTT Symposium
Number207
ISSN0357-9387

Conference

ConferenceEuropean Symposium on Enzymes in Grain Processing, ESEGP-2
Abbreviated titleESEGP-2
CountryFinland
CityHelsinki
Period8/12/9910/12/99

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Schmidt, A., Bailey, M., Vaari, A., Thomsen, A., & Tenkanen, M. (2000). Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. In 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2 (pp. 267-271). VTT Technical Research Centre of Finland. VTT Symposium, No. 207 http://www.vtt.fi/inf/pdf/symposiums/2000/S207.pdf