Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan

A. Schmidt, Michael Bailey, Anu Vaari, A. Thomsen, Maija Tenkanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

1 Citation (Scopus)

Abstract

In Denmark, the wet-oxidation (WO) process has been used for fractionating wheat straw [10], where most of the hemicellulose is solubilised mainly as oligomers and polymers and the cellulose retained as a solid. In wheat straw, hemicellulose is an arabino-4-O-methylglucurono xylan, which, in addition to xylose, contains arabinose, 4-O-Me-D-glucuronic acid, and acetic acid substituents [9]. Some arabinosyl groups may be esterified with ferulic or p-coumaric acids. Most micro-organisms used for lactate, ethanol or xylitol production do not produce enzymes for hemicellulose hydrolysis. Acid or enzymatic hydrolysis is needed to convert the sugar polymers to monomers. A problem associated with dilute acid hydrolysis is the poor fermentability of the produced hydrolysates compared with enzymatic hydrolysis. This study investigated the utilisation of wet-oxidised wheat straw xylan (WO-xylan) for production of enzymes for hydrolysis. Three fungal strains (Aspergillus oryzae, Aspergillus fumigatus, and Trichoderma reesei) were cultivated on WO-xylan using xylan (Lenzig AG) as a supplementary carbon-source. A. oryzae was selected for production of enzymes in larger scale.
Original languageEnglish
Title of host publication2nd European Symposium on Enzymes in Grain Processing, ESEPG-2
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages267-271
ISBN (Electronic)951-38-5707-7
ISBN (Print)951-38-5706-9
Publication statusPublished - 2000
MoE publication typeA4 Article in a conference publication
EventEuropean Symposium on Enzymes in Grain Processing, ESEGP-2 - Helsinki, Finland
Duration: 8 Dec 199910 Dec 1999

Publication series

NameVTT Symposium
PublisherVTT
Number207
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

ConferenceEuropean Symposium on Enzymes in Grain Processing, ESEGP-2
Abbreviated titleESEGP-2
CountryFinland
CityHelsinki
Period8/12/9910/12/99

Fingerprint

xylan
wheat straw
hemicellulose
cell walls
Aspergillus oryzae
acid hydrolysis
enzymatic hydrolysis
enzymes
polymers
hydrolysis
oxidation
Trichoderma reesei
xylitol
glucuronic acid
Aspergillus fumigatus
p-coumaric acid
arabinose
xylose
hydrolysates
Denmark

Cite this

Schmidt, A., Bailey, M., Vaari, A., Thomsen, A., & Tenkanen, M. (2000). Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. In 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2 (pp. 267-271). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 207
Schmidt, A. ; Bailey, Michael ; Vaari, Anu ; Thomsen, A. ; Tenkanen, Maija. / Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo : VTT Technical Research Centre of Finland, 2000. pp. 267-271 (VTT Symposium; No. 207).
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abstract = "In Denmark, the wet-oxidation (WO) process has been used for fractionating wheat straw [10], where most of the hemicellulose is solubilised mainly as oligomers and polymers and the cellulose retained as a solid. In wheat straw, hemicellulose is an arabino-4-O-methylglucurono xylan, which, in addition to xylose, contains arabinose, 4-O-Me-D-glucuronic acid, and acetic acid substituents [9]. Some arabinosyl groups may be esterified with ferulic or p-coumaric acids. Most micro-organisms used for lactate, ethanol or xylitol production do not produce enzymes for hemicellulose hydrolysis. Acid or enzymatic hydrolysis is needed to convert the sugar polymers to monomers. A problem associated with dilute acid hydrolysis is the poor fermentability of the produced hydrolysates compared with enzymatic hydrolysis. This study investigated the utilisation of wet-oxidised wheat straw xylan (WO-xylan) for production of enzymes for hydrolysis. Three fungal strains (Aspergillus oryzae, Aspergillus fumigatus, and Trichoderma reesei) were cultivated on WO-xylan using xylan (Lenzig AG) as a supplementary carbon-source. A. oryzae was selected for production of enzymes in larger scale.",
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Schmidt, A, Bailey, M, Vaari, A, Thomsen, A & Tenkanen, M 2000, Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. in 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 207, pp. 267-271, European Symposium on Enzymes in Grain Processing, ESEGP-2, Helsinki, Finland, 8/12/99.

Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. / Schmidt, A.; Bailey, Michael; Vaari, Anu; Thomsen, A.; Tenkanen, Maija.

2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo : VTT Technical Research Centre of Finland, 2000. p. 267-271 (VTT Symposium; No. 207).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

TY - GEN

T1 - Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan

AU - Schmidt, A.

AU - Bailey, Michael

AU - Vaari, Anu

AU - Thomsen, A.

AU - Tenkanen, Maija

PY - 2000

Y1 - 2000

N2 - In Denmark, the wet-oxidation (WO) process has been used for fractionating wheat straw [10], where most of the hemicellulose is solubilised mainly as oligomers and polymers and the cellulose retained as a solid. In wheat straw, hemicellulose is an arabino-4-O-methylglucurono xylan, which, in addition to xylose, contains arabinose, 4-O-Me-D-glucuronic acid, and acetic acid substituents [9]. Some arabinosyl groups may be esterified with ferulic or p-coumaric acids. Most micro-organisms used for lactate, ethanol or xylitol production do not produce enzymes for hemicellulose hydrolysis. Acid or enzymatic hydrolysis is needed to convert the sugar polymers to monomers. A problem associated with dilute acid hydrolysis is the poor fermentability of the produced hydrolysates compared with enzymatic hydrolysis. This study investigated the utilisation of wet-oxidised wheat straw xylan (WO-xylan) for production of enzymes for hydrolysis. Three fungal strains (Aspergillus oryzae, Aspergillus fumigatus, and Trichoderma reesei) were cultivated on WO-xylan using xylan (Lenzig AG) as a supplementary carbon-source. A. oryzae was selected for production of enzymes in larger scale.

AB - In Denmark, the wet-oxidation (WO) process has been used for fractionating wheat straw [10], where most of the hemicellulose is solubilised mainly as oligomers and polymers and the cellulose retained as a solid. In wheat straw, hemicellulose is an arabino-4-O-methylglucurono xylan, which, in addition to xylose, contains arabinose, 4-O-Me-D-glucuronic acid, and acetic acid substituents [9]. Some arabinosyl groups may be esterified with ferulic or p-coumaric acids. Most micro-organisms used for lactate, ethanol or xylitol production do not produce enzymes for hemicellulose hydrolysis. Acid or enzymatic hydrolysis is needed to convert the sugar polymers to monomers. A problem associated with dilute acid hydrolysis is the poor fermentability of the produced hydrolysates compared with enzymatic hydrolysis. This study investigated the utilisation of wet-oxidised wheat straw xylan (WO-xylan) for production of enzymes for hydrolysis. Three fungal strains (Aspergillus oryzae, Aspergillus fumigatus, and Trichoderma reesei) were cultivated on WO-xylan using xylan (Lenzig AG) as a supplementary carbon-source. A. oryzae was selected for production of enzymes in larger scale.

M3 - Conference article in proceedings

SN - 951-38-5706-9

T3 - VTT Symposium

SP - 267

EP - 271

BT - 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Schmidt A, Bailey M, Vaari A, Thomsen A, Tenkanen M. Production of fungal plant cell wall degrading enzymes on wet-oxidised wheat straw xylan. In 2nd European Symposium on Enzymes in Grain Processing, ESEPG-2. Espoo: VTT Technical Research Centre of Finland. 2000. p. 267-271. (VTT Symposium; No. 207).