Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5

Jacob Dam Sorensen, Evamaria I. Petersen, Marilyn Wiebe

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
Original languageEnglish
Pages (from-to)1227-1232
JournalBiotechnology Letters
Volume29
Issue number8
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • Cutinase
  • Fusarium venenatum
  • Recombinant protein

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