Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
- Fusarium venenatum
- Recombinant protein
Sorensen, J. D., Petersen, E. I., & Wiebe, M. (2007). Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5. Biotechnology Letters, 29(8), 1227-1232. https://doi.org/10.1007/s10529-007-9369-7