Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5

Jacob Dam Sorensen, Evamaria I. Petersen, Marilyn Wiebe

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
Original languageEnglish
Pages (from-to)1227-1232
JournalBiotechnology Letters
Volume29
Issue number8
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Glucan 1,4-alpha-Glucosidase
Aspergillus niger
Fusarium
Genes
Aspergillus
Starch
Glucose
Carbon
Plasmids
cutinase

Keywords

  • Cutinase
  • Fusarium venenatum
  • Recombinant protein

Cite this

Sorensen, Jacob Dam ; Petersen, Evamaria I. ; Wiebe, Marilyn. / Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5. In: Biotechnology Letters. 2007 ; Vol. 29, No. 8. pp. 1227-1232.
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abstract = "Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.",
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Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5. / Sorensen, Jacob Dam; Petersen, Evamaria I.; Wiebe, Marilyn.

In: Biotechnology Letters, Vol. 29, No. 8, 2007, p. 1227-1232.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5

AU - Sorensen, Jacob Dam

AU - Petersen, Evamaria I.

AU - Wiebe, Marilyn

PY - 2007

Y1 - 2007

N2 - Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.

AB - Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l−1 in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l−1 or 8 mg l−1) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.

KW - Cutinase

KW - Fusarium venenatum

KW - Recombinant protein

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JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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