Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase

Anne Usvalampi (Corresponding Author), Ossi Turunen, Jarkko Valjakka, Ossi Pastinen, Matti Leisola, Antti Nyyssölä

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia colil-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis–Menten kinetics for l-xylulose with a Km of 41 mM and a Vmax of 0.23 μmol/(mg min). The half-lives determined for the enzyme at 35 °C and at 45 °C were 6 h 50 min and 1 h 31 min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35 °C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14]l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.
Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalEnzyme and Microbial Technology
Volume50
Issue number1
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Xylulose
Isomerases
Xylose
Fucose
Escherichia coli
Xylitol
Enzymes
Escherichia
Rhamnose
Raw materials
Catalysts
Kinetics

Keywords

  • L-Xylose
  • L-Xylulose
  • L-Fucose isomerase
  • Isomerization
  • Rare sugars

Cite this

Usvalampi, Anne ; Turunen, Ossi ; Valjakka, Jarkko ; Pastinen, Ossi ; Leisola, Matti ; Nyyssölä, Antti. / Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase. In: Enzyme and Microbial Technology. 2012 ; Vol. 50, No. 1. pp. 71-76.
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Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase. / Usvalampi, Anne (Corresponding Author); Turunen, Ossi; Valjakka, Jarkko; Pastinen, Ossi; Leisola, Matti; Nyyssölä, Antti.

In: Enzyme and Microbial Technology, Vol. 50, No. 1, 2012, p. 71-76.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase

AU - Usvalampi, Anne

AU - Turunen, Ossi

AU - Valjakka, Jarkko

AU - Pastinen, Ossi

AU - Leisola, Matti

AU - Nyyssölä, Antti

PY - 2012

Y1 - 2012

N2 - l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia colil-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis–Menten kinetics for l-xylulose with a Km of 41 mM and a Vmax of 0.23 μmol/(mg min). The half-lives determined for the enzyme at 35 °C and at 45 °C were 6 h 50 min and 1 h 31 min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35 °C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14]l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.

AB - l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia colil-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis–Menten kinetics for l-xylulose with a Km of 41 mM and a Vmax of 0.23 μmol/(mg min). The half-lives determined for the enzyme at 35 °C and at 45 °C were 6 h 50 min and 1 h 31 min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35 °C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14]l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.

KW - L-Xylose

KW - L-Xylulose

KW - L-Fucose isomerase

KW - Isomerization

KW - Rare sugars

U2 - 10.1016/j.enzmictec.2011.09.009

DO - 10.1016/j.enzmictec.2011.09.009

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