Production of nisin with continuous adsorption to Amberlite XAD-4 resin using Lactococcus lactis N8 and L. lactis LAC48

M. Tolonen, P. E. J. Saris (Corresponding Author), Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

The production of nisin, biomass and lactic acid in pH-controlled and uncontrolled batch fermentation and batch fermentation (pH 5.5) with continuous removal of nisin was examined in the parent strain Lactococcus lactis N8 and LAC48. Strain LAC48 in batch fermentor (pH not controlled) gave a maximum nisin concentration of 2.5×106 IU g dcw−1. The nisin concentration remained high (2.0×106 IU g dcw−1) after the logarithmic growth phase (10–22 h), whereas nisin production of strain N8 decreased after the logarithmic growth phase. The maximum nisin production of strain LAC48 was not directly related to the biomass formation and not associated with growth. In order to study end product inhibition in nisin production, a system was built for adsorption of nisin during fermentation. The adsorbent Amberlite XAD-4 was found to have an effective binding capacity for nisin. Cells of LAC48 and N8 compensated for the removal of nisin, indicating that nisin production also occurs in the stationary phase.
Original languageEnglish
Pages (from-to)659 - 665
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume63
Issue number6
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Nisin
Lactococcus lactis
Adsorption
Fermentation
Biomass
XAD-4 resin
Growth
Bioreactors
Lactic Acid

Keywords

  • nisin
  • Lactococcus lactis
  • food preservative
  • preservatives

Cite this

@article{eff3b99d5fe342c3b233ab0b94ae0dc7,
title = "Production of nisin with continuous adsorption to Amberlite XAD-4 resin using Lactococcus lactis N8 and L. lactis LAC48",
abstract = "The production of nisin, biomass and lactic acid in pH-controlled and uncontrolled batch fermentation and batch fermentation (pH 5.5) with continuous removal of nisin was examined in the parent strain Lactococcus lactis N8 and LAC48. Strain LAC48 in batch fermentor (pH not controlled) gave a maximum nisin concentration of 2.5×106 IU g dcw−1. The nisin concentration remained high (2.0×106 IU g dcw−1) after the logarithmic growth phase (10–22 h), whereas nisin production of strain N8 decreased after the logarithmic growth phase. The maximum nisin production of strain LAC48 was not directly related to the biomass formation and not associated with growth. In order to study end product inhibition in nisin production, a system was built for adsorption of nisin during fermentation. The adsorbent Amberlite XAD-4 was found to have an effective binding capacity for nisin. Cells of LAC48 and N8 compensated for the removal of nisin, indicating that nisin production also occurs in the stationary phase.",
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author = "M. Tolonen and Saris, {P. E. J.} and Matti Siika-aho",
year = "2004",
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language = "English",
volume = "63",
pages = "659 -- 665",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
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Production of nisin with continuous adsorption to Amberlite XAD-4 resin using Lactococcus lactis N8 and L. lactis LAC48. / Tolonen, M.; Saris, P. E. J. (Corresponding Author); Siika-aho, Matti.

In: Applied Microbiology and Biotechnology, Vol. 63, No. 6, 2004, p. 659 - 665.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of nisin with continuous adsorption to Amberlite XAD-4 resin using Lactococcus lactis N8 and L. lactis LAC48

AU - Tolonen, M.

AU - Saris, P. E. J.

AU - Siika-aho, Matti

PY - 2004

Y1 - 2004

N2 - The production of nisin, biomass and lactic acid in pH-controlled and uncontrolled batch fermentation and batch fermentation (pH 5.5) with continuous removal of nisin was examined in the parent strain Lactococcus lactis N8 and LAC48. Strain LAC48 in batch fermentor (pH not controlled) gave a maximum nisin concentration of 2.5×106 IU g dcw−1. The nisin concentration remained high (2.0×106 IU g dcw−1) after the logarithmic growth phase (10–22 h), whereas nisin production of strain N8 decreased after the logarithmic growth phase. The maximum nisin production of strain LAC48 was not directly related to the biomass formation and not associated with growth. In order to study end product inhibition in nisin production, a system was built for adsorption of nisin during fermentation. The adsorbent Amberlite XAD-4 was found to have an effective binding capacity for nisin. Cells of LAC48 and N8 compensated for the removal of nisin, indicating that nisin production also occurs in the stationary phase.

AB - The production of nisin, biomass and lactic acid in pH-controlled and uncontrolled batch fermentation and batch fermentation (pH 5.5) with continuous removal of nisin was examined in the parent strain Lactococcus lactis N8 and LAC48. Strain LAC48 in batch fermentor (pH not controlled) gave a maximum nisin concentration of 2.5×106 IU g dcw−1. The nisin concentration remained high (2.0×106 IU g dcw−1) after the logarithmic growth phase (10–22 h), whereas nisin production of strain N8 decreased after the logarithmic growth phase. The maximum nisin production of strain LAC48 was not directly related to the biomass formation and not associated with growth. In order to study end product inhibition in nisin production, a system was built for adsorption of nisin during fermentation. The adsorbent Amberlite XAD-4 was found to have an effective binding capacity for nisin. Cells of LAC48 and N8 compensated for the removal of nisin, indicating that nisin production also occurs in the stationary phase.

KW - nisin

KW - Lactococcus lactis

KW - food preservative

KW - preservatives

U2 - 10.1007/s00253-003-1413-5

DO - 10.1007/s00253-003-1413-5

M3 - Article

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SP - 659

EP - 665

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

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