Production of proteases by Fusarium species grown on barley grains and in media containing cereal proteins

Anja Pekkarinen, Leena Mannonen, B. Jones, Marja-Leena Niku-Paavola

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

The production of proteases by the cereal plant pathogens Fusarium culmorum, F. graminearum and F. poae was followed through seven days of cultivation. The fungi were grown in mineral and in gluten culture media, and on autoclaved barley grains. The proteolytic activities of each sample were analysed at pH 2·2, 5·0 and 8·0 and the pH optima of the most active proteases were determined. All of the fungi grown in the gluten medium produced proteases that were active at pH levels between 6 and 10 and were most active at about pH 9·0. Fusarium poae also produced acid protease(s) with pH optima between 3.0 and 3.5 when grown in the gluten medium. No protease activity was detected in the cultures that were grown in the mineral medium, except that a small amount was formed after the glucose substrate was depleted. When grown on the barley grain medium the Fusarium species produced protease activities that were similar to the neutral and alkaline ones present in the gluten cultures, but no pH 2·2 protease activity was detected. The alkaline proteases had some characteristics that were similar to those of chymotrypsin.
Original languageEnglish
Pages (from-to)253 - 261
Number of pages9
JournalJournal of Cereal Science
Volume31
Issue number3
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

cereal proteins
Fusarium
Hordeum
Peptide Hydrolases
proteinases
barley
Glutens
gluten
Proteins
Fusarium poae
Fungi
Minerals
minerals
Chymotrypsin
Pathogens
Fusarium culmorum
fungi
Edible Grain
Fusarium graminearum
chymotrypsin

Keywords

  • Fusarium
  • protease
  • cereals
  • chymotrypsin

Cite this

Pekkarinen, Anja ; Mannonen, Leena ; Jones, B. ; Niku-Paavola, Marja-Leena. / Production of proteases by Fusarium species grown on barley grains and in media containing cereal proteins. In: Journal of Cereal Science. 2000 ; Vol. 31, No. 3. pp. 253 - 261.
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abstract = "The production of proteases by the cereal plant pathogens Fusarium culmorum, F. graminearum and F. poae was followed through seven days of cultivation. The fungi were grown in mineral and in gluten culture media, and on autoclaved barley grains. The proteolytic activities of each sample were analysed at pH 2·2, 5·0 and 8·0 and the pH optima of the most active proteases were determined. All of the fungi grown in the gluten medium produced proteases that were active at pH levels between 6 and 10 and were most active at about pH 9·0. Fusarium poae also produced acid protease(s) with pH optima between 3.0 and 3.5 when grown in the gluten medium. No protease activity was detected in the cultures that were grown in the mineral medium, except that a small amount was formed after the glucose substrate was depleted. When grown on the barley grain medium the Fusarium species produced protease activities that were similar to the neutral and alkaline ones present in the gluten cultures, but no pH 2·2 protease activity was detected. The alkaline proteases had some characteristics that were similar to those of chymotrypsin.",
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Production of proteases by Fusarium species grown on barley grains and in media containing cereal proteins. / Pekkarinen, Anja; Mannonen, Leena; Jones, B.; Niku-Paavola, Marja-Leena.

In: Journal of Cereal Science, Vol. 31, No. 3, 2000, p. 253 - 261.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The production of proteases by the cereal plant pathogens Fusarium culmorum, F. graminearum and F. poae was followed through seven days of cultivation. The fungi were grown in mineral and in gluten culture media, and on autoclaved barley grains. The proteolytic activities of each sample were analysed at pH 2·2, 5·0 and 8·0 and the pH optima of the most active proteases were determined. All of the fungi grown in the gluten medium produced proteases that were active at pH levels between 6 and 10 and were most active at about pH 9·0. Fusarium poae also produced acid protease(s) with pH optima between 3.0 and 3.5 when grown in the gluten medium. No protease activity was detected in the cultures that were grown in the mineral medium, except that a small amount was formed after the glucose substrate was depleted. When grown on the barley grain medium the Fusarium species produced protease activities that were similar to the neutral and alkaline ones present in the gluten cultures, but no pH 2·2 protease activity was detected. The alkaline proteases had some characteristics that were similar to those of chymotrypsin.

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