Production of protoplasts from the fungi Curvularia inaequalis and Trichoderma reesei

Heikki Laurila, Helena Nevalainen, Veijo Mäkinen

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Protoplast formation in Curvularia inaequalis was achieved using non-commercial and commercial snail gut enzymes or Trichoderma harzianum enzymes. The cells were grown for enzyme treatment on cellophane sheets or in liquid cultures for varying periods of time. The production of T. harzianum enzymes is discussed. The highest protoplast yields were 2.6x107 protoplasts/ml enzyme solution. Protoplasts were shown to have zero to four nuclei. Protoplast regeneration was succesfully carried out in semisolid agar.

Original languageEnglish
Pages (from-to)210 - 212
Number of pages3
JournalApplied Microbiology and Biotechnology
Volume21
Issue number3-4
DOIs
Publication statusPublished - 1985
MoE publication typeNot Eligible

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Trichoderma
Protoplasts
Fungi
Enzymes
Cellophane
Snails
Agar
Regeneration

Cite this

Laurila, Heikki ; Nevalainen, Helena ; Mäkinen, Veijo. / Production of protoplasts from the fungi Curvularia inaequalis and Trichoderma reesei. In: Applied Microbiology and Biotechnology. 1985 ; Vol. 21, No. 3-4. pp. 210 - 212.
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Production of protoplasts from the fungi Curvularia inaequalis and Trichoderma reesei. / Laurila, Heikki; Nevalainen, Helena; Mäkinen, Veijo.

In: Applied Microbiology and Biotechnology, Vol. 21, No. 3-4, 1985, p. 210 - 212.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Production of protoplasts from the fungi Curvularia inaequalis and Trichoderma reesei

AU - Laurila, Heikki

AU - Nevalainen, Helena

AU - Mäkinen, Veijo

PY - 1985

Y1 - 1985

N2 - Protoplast formation in Curvularia inaequalis was achieved using non-commercial and commercial snail gut enzymes or Trichoderma harzianum enzymes. The cells were grown for enzyme treatment on cellophane sheets or in liquid cultures for varying periods of time. The production of T. harzianum enzymes is discussed. The highest protoplast yields were 2.6x107 protoplasts/ml enzyme solution. Protoplasts were shown to have zero to four nuclei. Protoplast regeneration was succesfully carried out in semisolid agar.

AB - Protoplast formation in Curvularia inaequalis was achieved using non-commercial and commercial snail gut enzymes or Trichoderma harzianum enzymes. The cells were grown for enzyme treatment on cellophane sheets or in liquid cultures for varying periods of time. The production of T. harzianum enzymes is discussed. The highest protoplast yields were 2.6x107 protoplasts/ml enzyme solution. Protoplasts were shown to have zero to four nuclei. Protoplast regeneration was succesfully carried out in semisolid agar.

U2 - 10.1007/BF00295124

DO - 10.1007/BF00295124

M3 - Article

VL - 21

SP - 210

EP - 212

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

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