Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 539 - 552 |
| Number of pages | 14 |
| Journal | Phytochemistry Reviews |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Allergy
- Expression system
- Green biotechnology
- Molecular farming
- Recombinant protein
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Production of recombinant allergens in plants. / Schmidt, G.; Gadermaier, G.; Pertl, H.; Siegert, M.; Oksman-Caldentey, Kirsi-Marja; Ritala, Anneli; Himly, M.; Obermeyer, G.; Ferreira, F. (Corresponding Author).
In: Phytochemistry Reviews, Vol. 7, No. 3, 2008, p. 539 - 552.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Production of recombinant allergens in plants
AU - Schmidt, G.
AU - Gadermaier, G.
AU - Pertl, H.
AU - Siegert, M.
AU - Oksman-Caldentey, Kirsi-Marja
AU - Ritala, Anneli
AU - Himly, M.
AU - Obermeyer, G.
AU - Ferreira, F.
PY - 2008
Y1 - 2008
N2 - A large percentage of allergenic proteins are of plant origin. Hence, plant-based expression systems are considered ideal for the recombinant production of certain allergens. First attempts to establish production of plant-derived allergens in plants focused on transient expression in Nicotiana benthamiana infected with recombinant viral vectors. Accordingly, allergens from birch and mugwort pollen, as well as from apple have been expressed in plants. Production of house dust mite allergens has been achieved by Agrobacterium-mediated transformation of tobacco plants. Beside the use of plants as production systems, other approaches have focused on the development of edible vaccines expressing allergens or epitopes thereof, which bypasses the need of allergen purification. The potential of this approach has been convincingly demonstrated for transgenic rice seeds expressing seven dominant human T cell epitopes derived from Japanese cedar pollen allergens. Parallel to efforts in developing recombinant-based diagnostic and therapeutic reagents, different gene-silencing approaches have been used to decrease the expression of allergenic proteins in allergen sources. In this way hypoallergenic ryegrass, soybean, rice, apple, and tomato were developed.
AB - A large percentage of allergenic proteins are of plant origin. Hence, plant-based expression systems are considered ideal for the recombinant production of certain allergens. First attempts to establish production of plant-derived allergens in plants focused on transient expression in Nicotiana benthamiana infected with recombinant viral vectors. Accordingly, allergens from birch and mugwort pollen, as well as from apple have been expressed in plants. Production of house dust mite allergens has been achieved by Agrobacterium-mediated transformation of tobacco plants. Beside the use of plants as production systems, other approaches have focused on the development of edible vaccines expressing allergens or epitopes thereof, which bypasses the need of allergen purification. The potential of this approach has been convincingly demonstrated for transgenic rice seeds expressing seven dominant human T cell epitopes derived from Japanese cedar pollen allergens. Parallel to efforts in developing recombinant-based diagnostic and therapeutic reagents, different gene-silencing approaches have been used to decrease the expression of allergenic proteins in allergen sources. In this way hypoallergenic ryegrass, soybean, rice, apple, and tomato were developed.
KW - Allergy
KW - Expression system
KW - Green biotechnology
KW - Molecular farming
KW - Recombinant protein
U2 - 10.1007/s11101-008-9099-z
DO - 10.1007/s11101-008-9099-z
M3 - Article
VL - 7
SP - 539
EP - 552
JO - Phytochemistry Reviews
JF - Phytochemistry Reviews
SN - 1568-7767
IS - 3
ER -