Production of recombinant gelatin in transgenic barley grain

Anneli Ritala, K. Eskelin, Heidi Holkeri, E. Wahlström, J. Baez, K. Mäkinen, Anna-Maria Nuutila

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages132-132
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0
Publication statusPublished - 2007
MoE publication typeNot Eligible
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

SeriesVTT Symposium
Number249
ISSN0357-9387

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

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