Production of recombinant gelatin in transgenic barley grain

Anneli Ritala, K. Eskelin, Heidi Holkeri, E. Wahlström, J. Baez, K. Mäkinen, Anna-Maria Nuutila

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific


    The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.
    Original languageEnglish
    Title of host publicationPlants for Human Health in the Post-Genome Era
    Subtitle of host publicationPSE Congress
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    ISBN (Electronic)978-951-38-6322-7
    ISBN (Print)978-951-38-6321-0
    Publication statusPublished - 2007
    MoE publication typeNot Eligible
    EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
    Duration: 26 Aug 200729 Aug 2007

    Publication series

    SeriesVTT Symposium


    ConferencePSE Congress: Plants for Human Health in the Post-Genome Era


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