Production of recombinant gelatin in transgenic barley grain

Anneli Ritala, K. Eskelin, Heidi Holkeri, E. Wahlström, J. Baez, K. Mäkinen, Anna-Maria Nuutila

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages132-132
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0
Publication statusPublished - 2007
MoE publication typeNot Eligible
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

SeriesVTT Symposium
Number249
ISSN0357-9387

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

Fingerprint

gelatin
barley
genetically modified organisms
promoter regions
glutelins
recombinant DNA
ubiquitin
plant proteins
Liliopsida
signal peptide
Agrobacterium
amylases
protein sources
collagen
animals
embryo (animal)
production technology
immatures
germination
rice

Cite this

Ritala, A., Eskelin, K., Holkeri, H., Wahlström, E., Baez, J., Mäkinen, K., & Nuutila, A-M. (2007). Production of recombinant gelatin in transgenic barley grain. In Plants for Human Health in the Post-Genome Era: PSE Congress (pp. 132-132). [E8] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 249
Ritala, Anneli ; Eskelin, K. ; Holkeri, Heidi ; Wahlström, E. ; Baez, J. ; Mäkinen, K. ; Nuutila, Anna-Maria. / Production of recombinant gelatin in transgenic barley grain. Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 132-132 (VTT Symposium; No. 249).
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Ritala, A, Eskelin, K, Holkeri, H, Wahlström, E, Baez, J, Mäkinen, K & Nuutila, A-M 2007, Production of recombinant gelatin in transgenic barley grain. in Plants for Human Health in the Post-Genome Era: PSE Congress., E8, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 249, pp. 132-132, PSE Congress: Plants for Human Health in the Post-Genome Era, Helsinki, Finland, 26/08/07.

Production of recombinant gelatin in transgenic barley grain. / Ritala, Anneli; Eskelin, K.; Holkeri, Heidi; Wahlström, E.; Baez, J.; Mäkinen, K.; Nuutila, Anna-Maria.

Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. p. 132-132 E8 (VTT Symposium; No. 249).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Production of recombinant gelatin in transgenic barley grain

AU - Ritala, Anneli

AU - Eskelin, K.

AU - Holkeri, Heidi

AU - Wahlström, E.

AU - Baez, J.

AU - Mäkinen, K.

AU - Nuutila, Anna-Maria

PY - 2007

Y1 - 2007

N2 - The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.

AB - The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.

M3 - Conference abstract in proceedings

SN - 978-951-38-6321-0

T3 - VTT Symposium

SP - 132

EP - 132

BT - Plants for Human Health in the Post-Genome Era

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Ritala A, Eskelin K, Holkeri H, Wahlström E, Baez J, Mäkinen K et al. Production of recombinant gelatin in transgenic barley grain. In Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo: VTT Technical Research Centre of Finland. 2007. p. 132-132. E8. (VTT Symposium; No. 249).