@inbook{9c2d67a29028486ca6f3be0552a2b330,
title = "Production of recombinant gelatin in transgenic barley grain",
abstract = "The large-scale production of recombinant DNA-based mammalian proteins in plants can provide a safe, animal-component free, homogenous and cost-effective source for those proteins that are currently derived from animal or human sources. The aim of our project is to develop a production system for an industrial protein, gelatin, utilizing barley grain. Gelatin is an important component in many products in the food, photographic, and pharmaceutical industries. In this study a 50,000 Dalton fragment of the human collagen I alpha 1 chain was accumulated in transgenic barley grain. The monocot expression optimized cDNA coding for this recombinant gelatin was fused to a signal sequence and the ER retention signal HDEL. Gene expression was controlled under (1) a constitutive maize ubiquitin promoter, (2) a seed-specific rice glutelin promoter or (3) a germination dependent barley -amylase promoter. The gene constructs were co-transformed with the bar or hyg selection marker genes into immature barley embryos either by particle bombardment or by Agrobacterium-mediated delivery. The accumulation levels of the recombinant gelatin in the barley grains varied depending on the promoter used.",
author = "Anneli Ritala and K. Eskelin and Heidi Holkeri and E. Wahlstr{\"o}m and J. Baez and K. M{\"a}kinen and Anna-Maria Nuutila",
year = "2007",
language = "English",
isbn = "978-951-38-6321-0",
series = "VTT Symposium",
publisher = "VTT Technical Research Centre of Finland",
number = "249",
pages = "132--132",
booktitle = "Plants for Human Health in the Post-Genome Era",
address = "Finland",
note = "PSE Congress: Plants for Human Health in the Post-Genome Era ; Conference date: 26-08-2007 Through 29-08-2007",
}