Production of recombinant proteins in the filamentous fungus Trichoderma reesei

Sirkka Keränen, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

68 Citations (Scopus)

Abstract

The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition the strong inducible cbh 1 promoter, other promoters, which are regulated in a different manner, have been developed.

Original languageEnglish
Pages (from-to)534-537
Number of pages4
JournalCurrent Opinion in Biotechnology
Volume6
Issue number5
DOIs
Publication statusPublished - 1 Jan 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Recombinant proteins
Trichoderma
Fungi
Recombinant Proteins
Proteins
Cellulose 1,4-beta-Cellobiosidase
Immunoglobulin Fragments
Immunoglobulin Fab Fragments
Cellulase
Antibodies

Cite this

@article{1412f263a14f48068cdff33944e85c64,
title = "Production of recombinant proteins in the filamentous fungus Trichoderma reesei",
abstract = "The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition the strong inducible cbh 1 promoter, other promoters, which are regulated in a different manner, have been developed.",
author = "Sirkka Ker{\"a}nen and Merja Penttil{\"a}",
year = "1995",
month = "1",
day = "1",
doi = "10.1016/0958-1669(95)80088-3",
language = "English",
volume = "6",
pages = "534--537",
journal = "Current Opinion in Biotechnology",
issn = "0958-1669",
publisher = "Elsevier",
number = "5",

}

Production of recombinant proteins in the filamentous fungus Trichoderma reesei. / Keränen, Sirkka; Penttilä, Merja.

In: Current Opinion in Biotechnology, Vol. 6, No. 5, 01.01.1995, p. 534-537.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of recombinant proteins in the filamentous fungus Trichoderma reesei

AU - Keränen, Sirkka

AU - Penttilä, Merja

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition the strong inducible cbh 1 promoter, other promoters, which are regulated in a different manner, have been developed.

AB - The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition the strong inducible cbh 1 promoter, other promoters, which are regulated in a different manner, have been developed.

UR - http://www.scopus.com/inward/record.url?scp=0028877138&partnerID=8YFLogxK

U2 - 10.1016/0958-1669(95)80088-3

DO - 10.1016/0958-1669(95)80088-3

M3 - Article

C2 - 7579664

AN - SCOPUS:0028877138

VL - 6

SP - 534

EP - 537

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

IS - 5

ER -