Production of Trichoderma reesei cellulases on glucose-containing media

Research output: Contribution to journalArticleScientificpeer-review

73 Citations (Scopus)

Abstract

The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.
Original languageEnglish
Pages (from-to)3650-3655
JournalApplied and Environmental Microbiology
Volume61
Issue number10
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulases
Trichoderma reesei
Trichoderma
cellulases
Cellulose 1,4-beta-Cellobiosidase
cellulose 1,4-beta-cellobiosidase
glucose
Fungi
promoter regions
fungus
endo-1,4-beta-glucanase
Glucose
fungi
Catalytic Domain
Peptide Elongation Factor 1
protein
Culture Media
Proteins
culture media
protein synthesis

Cite this

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title = "Production of Trichoderma reesei cellulases on glucose-containing media",
abstract = "The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.",
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Production of Trichoderma reesei cellulases on glucose-containing media. / Nakari-Setälä, Tiina; Penttilä, Merja.

In: Applied and Environmental Microbiology, Vol. 61, No. 10, 1995, p. 3650-3655.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of Trichoderma reesei cellulases on glucose-containing media

AU - Nakari-Setälä, Tiina

AU - Penttilä, Merja

PY - 1995

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N2 - The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.

AB - The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.

M3 - Article

VL - 61

SP - 3650

EP - 3655

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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