Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

E. Dehghan (Corresponding Author), Suvi T. Häkkinen, Kirsi-Marja Oksman-Caldentey, F. Shahriari Ahmadi

Research output: Contribution to journalArticleScientificpeer-review

47 Citations (Scopus)

Abstract

In this study, the effects of ploidy level and culture medium were studied on the production of tropane alkaloids. We have successfully produced stable tetraploid hairy root lines of Hyoscyamus muticus and their ploidy stability was confirmed 30 months after transformation. Tetraploidy affected the growth rate and alkaloid accumulation in plants and transformed root cultures of Egyptian henbane. Although tetraploid plants could produce 200% higher scopolamine than their diploid counterparts, this result was not observed for corresponding induced hairy root cultures. Culture conditions did not only play an important role for biomass production, but also significantly affected tropane alkaloid accumulation in hairy root cultures. In spite of its lower biomass production, tetraploid clone could produce more scopolamine than the diploid counterpart under similar growth conditions. The highest yields of scopolamine (13.87 mg l−1) and hyoscyamine (107.7 mg 1−1) were obtained when diploid clones were grown on medium consisting of either Murashige and Skoog with 60 g/l sucrose or Gamborg’s B5 with 40 g/l sucrose, respectively. Although the hyoscyamine is the main alkaloid in the H. muticus plants, manipulation of ploidy level and culture conditions successfully changed the scopolamine/hyoscyamine ratio towards scopolamine. The fact that hyoscyamine is converted to scopolamine is very important due to the higher market value of scopolamine.
Original languageEnglish
Pages (from-to)35-44
JournalPlant Cell, Tissue and Organ Culture
Volume110
Issue number1
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Hyoscyamus muticus
tropane alkaloids
scopolamine
tetraploidy
diploidy
atropine
ploidy
alkaloids
biomass production
sucrose
clones
market value
culture media

Keywords

  • flow cytometry
  • GC-MS
  • hairy root cultures
  • Hyoscyamus muticus
  • ploidy level
  • tropane alkaloids

Cite this

@article{8813a2a0365b46589d02a2728858078a,
title = "Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)",
abstract = "In this study, the effects of ploidy level and culture medium were studied on the production of tropane alkaloids. We have successfully produced stable tetraploid hairy root lines of Hyoscyamus muticus and their ploidy stability was confirmed 30 months after transformation. Tetraploidy affected the growth rate and alkaloid accumulation in plants and transformed root cultures of Egyptian henbane. Although tetraploid plants could produce 200{\%} higher scopolamine than their diploid counterparts, this result was not observed for corresponding induced hairy root cultures. Culture conditions did not only play an important role for biomass production, but also significantly affected tropane alkaloid accumulation in hairy root cultures. In spite of its lower biomass production, tetraploid clone could produce more scopolamine than the diploid counterpart under similar growth conditions. The highest yields of scopolamine (13.87 mg l−1) and hyoscyamine (107.7 mg 1−1) were obtained when diploid clones were grown on medium consisting of either Murashige and Skoog with 60 g/l sucrose or Gamborg’s B5 with 40 g/l sucrose, respectively. Although the hyoscyamine is the main alkaloid in the H. muticus plants, manipulation of ploidy level and culture conditions successfully changed the scopolamine/hyoscyamine ratio towards scopolamine. The fact that hyoscyamine is converted to scopolamine is very important due to the higher market value of scopolamine.",
keywords = "flow cytometry, GC-MS, hairy root cultures, Hyoscyamus muticus, ploidy level, tropane alkaloids",
author = "E. Dehghan and H{\"a}kkinen, {Suvi T.} and Kirsi-Marja Oksman-Caldentey and {Shahriari Ahmadi}, F.",
year = "2012",
doi = "10.1007/s11240-012-0127-8",
language = "English",
volume = "110",
pages = "35--44",
journal = "Plant Cell, Tissue and Organ Culture",
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Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). / Dehghan, E. (Corresponding Author); Häkkinen, Suvi T.; Oksman-Caldentey, Kirsi-Marja; Shahriari Ahmadi, F.

In: Plant Cell, Tissue and Organ Culture, Vol. 110, No. 1, 2012, p. 35-44.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

AU - Dehghan, E.

AU - Häkkinen, Suvi T.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Shahriari Ahmadi, F.

PY - 2012

Y1 - 2012

N2 - In this study, the effects of ploidy level and culture medium were studied on the production of tropane alkaloids. We have successfully produced stable tetraploid hairy root lines of Hyoscyamus muticus and their ploidy stability was confirmed 30 months after transformation. Tetraploidy affected the growth rate and alkaloid accumulation in plants and transformed root cultures of Egyptian henbane. Although tetraploid plants could produce 200% higher scopolamine than their diploid counterparts, this result was not observed for corresponding induced hairy root cultures. Culture conditions did not only play an important role for biomass production, but also significantly affected tropane alkaloid accumulation in hairy root cultures. In spite of its lower biomass production, tetraploid clone could produce more scopolamine than the diploid counterpart under similar growth conditions. The highest yields of scopolamine (13.87 mg l−1) and hyoscyamine (107.7 mg 1−1) were obtained when diploid clones were grown on medium consisting of either Murashige and Skoog with 60 g/l sucrose or Gamborg’s B5 with 40 g/l sucrose, respectively. Although the hyoscyamine is the main alkaloid in the H. muticus plants, manipulation of ploidy level and culture conditions successfully changed the scopolamine/hyoscyamine ratio towards scopolamine. The fact that hyoscyamine is converted to scopolamine is very important due to the higher market value of scopolamine.

AB - In this study, the effects of ploidy level and culture medium were studied on the production of tropane alkaloids. We have successfully produced stable tetraploid hairy root lines of Hyoscyamus muticus and their ploidy stability was confirmed 30 months after transformation. Tetraploidy affected the growth rate and alkaloid accumulation in plants and transformed root cultures of Egyptian henbane. Although tetraploid plants could produce 200% higher scopolamine than their diploid counterparts, this result was not observed for corresponding induced hairy root cultures. Culture conditions did not only play an important role for biomass production, but also significantly affected tropane alkaloid accumulation in hairy root cultures. In spite of its lower biomass production, tetraploid clone could produce more scopolamine than the diploid counterpart under similar growth conditions. The highest yields of scopolamine (13.87 mg l−1) and hyoscyamine (107.7 mg 1−1) were obtained when diploid clones were grown on medium consisting of either Murashige and Skoog with 60 g/l sucrose or Gamborg’s B5 with 40 g/l sucrose, respectively. Although the hyoscyamine is the main alkaloid in the H. muticus plants, manipulation of ploidy level and culture conditions successfully changed the scopolamine/hyoscyamine ratio towards scopolamine. The fact that hyoscyamine is converted to scopolamine is very important due to the higher market value of scopolamine.

KW - flow cytometry

KW - GC-MS

KW - hairy root cultures

KW - Hyoscyamus muticus

KW - ploidy level

KW - tropane alkaloids

U2 - 10.1007/s11240-012-0127-8

DO - 10.1007/s11240-012-0127-8

M3 - Article

VL - 110

SP - 35

EP - 44

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 1

ER -