Production of xylooligosaccharides (XOS) from delignified sugarcane bagasse by peroxide-HAc process using recombinant xylanase from Bacillus subtilis

Juliano Bragatto, F. Segato (Corresponding Author), F.M. Squina

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

Xylan is a structural component of the plant cell wall, the most abundant hemicellulose in the nature and generally is considered as an agricultural waste. However, due to upcoming environmental changes, and the excess of residues generated by industrial process, sustainable methods are being applied in the waste management. The world interest in the utilization of agricultural residues as a source to produce biofuel and other products is increasing. Brazil is the biggest producer of sugarcane in the world generating around 110 million tons of dried bagasse. The hemicellulose in sugarcane bagasse is rich in xylan and can be used to produce xylose, xylitol, second-generation ethanol and xylooligosaccharides (XOS). This work, investigated the combination of the pre-treatment with hydrogen peroxide and acetic acid followed by the application of a recombinant xylanase from Bacillus subtilis to produce XOS from sugarcane bagasse. The main products released by the xylanase after the exposure of sugarcane bagasse to different periods of pre-treatment were xylotriose (X3), xylotetraose (X4), xylopentaose (X5) and also is less amounts xylooligomers up to 11 mers (X11), suggesting that is an endo-acting xylanase. The process yielded was 113 and 119 mg/g of xylooligomers from sugarcane bagasse pre-treated with peroxide-HAc for 7 and 8 h, respectively. The hydrolyzed xylooligomers were identified and quantified by HPAE-PAD validating the efficiency of both methods applied to XOS production from sugarcane bagasse.
Original languageEnglish
Pages (from-to)123-129
JournalIndustrial Crops and Products
Volume51
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

xylooligosaccharides
sugarcane bagasse
xylanases
peroxides
Bacillus subtilis
xylan
hemicellulose
pretreatment
xylitol
agricultural wastes
bagasse
waste management
xylose
biofuels
acetic acid
sugarcane
hydrogen peroxide
ethanol
cell walls
Brazil

Keywords

  • biomass
  • biorefinery
  • oligosaccharide
  • pretreatment
  • probiotics
  • recombinant protein

Cite this

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title = "Production of xylooligosaccharides (XOS) from delignified sugarcane bagasse by peroxide-HAc process using recombinant xylanase from Bacillus subtilis",
abstract = "Xylan is a structural component of the plant cell wall, the most abundant hemicellulose in the nature and generally is considered as an agricultural waste. However, due to upcoming environmental changes, and the excess of residues generated by industrial process, sustainable methods are being applied in the waste management. The world interest in the utilization of agricultural residues as a source to produce biofuel and other products is increasing. Brazil is the biggest producer of sugarcane in the world generating around 110 million tons of dried bagasse. The hemicellulose in sugarcane bagasse is rich in xylan and can be used to produce xylose, xylitol, second-generation ethanol and xylooligosaccharides (XOS). This work, investigated the combination of the pre-treatment with hydrogen peroxide and acetic acid followed by the application of a recombinant xylanase from Bacillus subtilis to produce XOS from sugarcane bagasse. The main products released by the xylanase after the exposure of sugarcane bagasse to different periods of pre-treatment were xylotriose (X3), xylotetraose (X4), xylopentaose (X5) and also is less amounts xylooligomers up to 11 mers (X11), suggesting that is an endo-acting xylanase. The process yielded was 113 and 119 mg/g of xylooligomers from sugarcane bagasse pre-treated with peroxide-HAc for 7 and 8 h, respectively. The hydrolyzed xylooligomers were identified and quantified by HPAE-PAD validating the efficiency of both methods applied to XOS production from sugarcane bagasse.",
keywords = "biomass, biorefinery, oligosaccharide, pretreatment, probiotics, recombinant protein",
author = "Juliano Bragatto and F. Segato and F.M. Squina",
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language = "English",
volume = "51",
pages = "123--129",
journal = "Industrial Crops and Products",
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Production of xylooligosaccharides (XOS) from delignified sugarcane bagasse by peroxide-HAc process using recombinant xylanase from Bacillus subtilis. / Bragatto, Juliano; Segato, F. (Corresponding Author); Squina, F.M.

In: Industrial Crops and Products, Vol. 51, 2013, p. 123-129.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Production of xylooligosaccharides (XOS) from delignified sugarcane bagasse by peroxide-HAc process using recombinant xylanase from Bacillus subtilis

AU - Bragatto, Juliano

AU - Segato, F.

AU - Squina, F.M.

PY - 2013

Y1 - 2013

N2 - Xylan is a structural component of the plant cell wall, the most abundant hemicellulose in the nature and generally is considered as an agricultural waste. However, due to upcoming environmental changes, and the excess of residues generated by industrial process, sustainable methods are being applied in the waste management. The world interest in the utilization of agricultural residues as a source to produce biofuel and other products is increasing. Brazil is the biggest producer of sugarcane in the world generating around 110 million tons of dried bagasse. The hemicellulose in sugarcane bagasse is rich in xylan and can be used to produce xylose, xylitol, second-generation ethanol and xylooligosaccharides (XOS). This work, investigated the combination of the pre-treatment with hydrogen peroxide and acetic acid followed by the application of a recombinant xylanase from Bacillus subtilis to produce XOS from sugarcane bagasse. The main products released by the xylanase after the exposure of sugarcane bagasse to different periods of pre-treatment were xylotriose (X3), xylotetraose (X4), xylopentaose (X5) and also is less amounts xylooligomers up to 11 mers (X11), suggesting that is an endo-acting xylanase. The process yielded was 113 and 119 mg/g of xylooligomers from sugarcane bagasse pre-treated with peroxide-HAc for 7 and 8 h, respectively. The hydrolyzed xylooligomers were identified and quantified by HPAE-PAD validating the efficiency of both methods applied to XOS production from sugarcane bagasse.

AB - Xylan is a structural component of the plant cell wall, the most abundant hemicellulose in the nature and generally is considered as an agricultural waste. However, due to upcoming environmental changes, and the excess of residues generated by industrial process, sustainable methods are being applied in the waste management. The world interest in the utilization of agricultural residues as a source to produce biofuel and other products is increasing. Brazil is the biggest producer of sugarcane in the world generating around 110 million tons of dried bagasse. The hemicellulose in sugarcane bagasse is rich in xylan and can be used to produce xylose, xylitol, second-generation ethanol and xylooligosaccharides (XOS). This work, investigated the combination of the pre-treatment with hydrogen peroxide and acetic acid followed by the application of a recombinant xylanase from Bacillus subtilis to produce XOS from sugarcane bagasse. The main products released by the xylanase after the exposure of sugarcane bagasse to different periods of pre-treatment were xylotriose (X3), xylotetraose (X4), xylopentaose (X5) and also is less amounts xylooligomers up to 11 mers (X11), suggesting that is an endo-acting xylanase. The process yielded was 113 and 119 mg/g of xylooligomers from sugarcane bagasse pre-treated with peroxide-HAc for 7 and 8 h, respectively. The hydrolyzed xylooligomers were identified and quantified by HPAE-PAD validating the efficiency of both methods applied to XOS production from sugarcane bagasse.

KW - biomass

KW - biorefinery

KW - oligosaccharide

KW - pretreatment

KW - probiotics

KW - recombinant protein

U2 - 10.1016/j.indcrop.2013.08.062

DO - 10.1016/j.indcrop.2013.08.062

M3 - Article

VL - 51

SP - 123

EP - 129

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -