Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene (pgk1) of Trichoderma reesei

Sirpa Vanhanen; Anu Saloheimo; Marja Ilmén; Jonathan K.C. Knowles; Merja Penttilä

doi:10.1016/0378-1119(91)90577-X

Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene (pgk1) of Trichoderma reesei

Sirpa Vanhanen, Anu Saloheimo, Marja Ilmén, Jonathan K.C. Knowles, Merja Penttilä

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Abstract

Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of Trichoderma reesei results in two transcripts due to two main transcription start points (tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putative regulatory elements present also in the PGK promoter of Saccharomyces cerevisiae: a 20-nt long sequence similar to the CTTCC-repeat region of the upstream activating sequence UAS, the eukaryotic heat-shock consensus sequence, HSE, and a putative eukaryotic cAMP regulatory sequence. The functionality of the putative HSE sequence was examined, but no clear effect could be seen on the total amount of pgk1 mRNA at elevated temperatures nor on transcription initiation from the upstream tsp, preceded by the HSE sequence.

Original language	English
Pages (from-to)	129-133
Journal	Gene
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90577-X
Publication status	Published - 30 Sept 1991
MoE publication type	A1 Journal article-refereed

Keywords

cAMP regulatory element
filamentous fungus
heat-shock element
Recombinant DNA
upstream activating sequence

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10.1016/0378-1119(91)90577-X

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abstract = "Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of Trichoderma reesei results in two transcripts due to two main transcription start points (tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putative regulatory elements present also in the PGK promoter of Saccharomyces cerevisiae: a 20-nt long sequence similar to the CTTCC-repeat region of the upstream activating sequence UAS, the eukaryotic heat-shock consensus sequence, HSE, and a putative eukaryotic cAMP regulatory sequence. The functionality of the putative HSE sequence was examined, but no clear effect could be seen on the total amount of pgk1 mRNA at elevated temperatures nor on transcription initiation from the upstream tsp, preceded by the HSE sequence.",

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AU - Vanhanen, Sirpa

AU - Saloheimo, Anu

AU - Ilmén, Marja

AU - Knowles, Jonathan K.C.

AU - Penttilä, Merja

PY - 1991/9/30

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AB - Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of Trichoderma reesei results in two transcripts due to two main transcription start points (tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putative regulatory elements present also in the PGK promoter of Saccharomyces cerevisiae: a 20-nt long sequence similar to the CTTCC-repeat region of the upstream activating sequence UAS, the eukaryotic heat-shock consensus sequence, HSE, and a putative eukaryotic cAMP regulatory sequence. The functionality of the putative HSE sequence was examined, but no clear effect could be seen on the total amount of pgk1 mRNA at elevated temperatures nor on transcription initiation from the upstream tsp, preceded by the HSE sequence.

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KW - filamentous fungus

KW - heat-shock element

KW - Recombinant DNA

KW - upstream activating sequence

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Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene (pgk1) of Trichoderma reesei

Abstract

Keywords

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