Protease-induced solubilisation of carbohydrates from brewers' spent grain

Craig B. Faulds, Samuel Collins, James A. Robertson, Janneke Treimo, Vincent G. H. Eijsink, Sandra W. A. Hinz, Henk A. Schols, Johanna Buchert, Keith W. Waldron

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose was derived from residual starch-derived products persisting in BSG after mashing. The proteases did not cleave the hydroxycinnamate ester linkages present on the arabinoxylan backbone, and thus do not behave as feruloyl esterases. However, the material solubilised from spent grain by the proteases contained up to 198 μg bound ferulic acid/g extract, which represented 8.6% of the total ferulic acid present in BSG. These results suggest that a portion of water-extractable feruloylated arabinoxylan and starch is trapped within the BSG matrix by a proteinaceous barrier.
Original languageEnglish
Pages (from-to)332-336
JournalJournal of Cereal Science
Volume50
Issue number3
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

spent grains
brewers grains
ferulic acid
solubilization
Peptide Hydrolases
proteinases
Carbohydrates
carbohydrates
arabinoxylan
Starch
Glucose
starch
mashing
Esters
glucose
Hydrolysis
Water
hydrolysis
esters
Proteins

Keywords

  • brewers
  • spent grain
  • starch
  • arabinoxylan
  • protease
  • cereal by-products

Cite this

Faulds, C. B., Collins, S., Robertson, J. A., Treimo, J., Eijsink, V. G. H., Hinz, S. W. A., ... Waldron, K. W. (2009). Protease-induced solubilisation of carbohydrates from brewers' spent grain. Journal of Cereal Science, 50(3), 332-336. https://doi.org/10.1016/j.jcs.2009.01.004
Faulds, Craig B. ; Collins, Samuel ; Robertson, James A. ; Treimo, Janneke ; Eijsink, Vincent G. H. ; Hinz, Sandra W. A. ; Schols, Henk A. ; Buchert, Johanna ; Waldron, Keith W. / Protease-induced solubilisation of carbohydrates from brewers' spent grain. In: Journal of Cereal Science. 2009 ; Vol. 50, No. 3. pp. 332-336.
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abstract = "The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50{\%} of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose was derived from residual starch-derived products persisting in BSG after mashing. The proteases did not cleave the hydroxycinnamate ester linkages present on the arabinoxylan backbone, and thus do not behave as feruloyl esterases. However, the material solubilised from spent grain by the proteases contained up to 198 μg bound ferulic acid/g extract, which represented 8.6{\%} of the total ferulic acid present in BSG. These results suggest that a portion of water-extractable feruloylated arabinoxylan and starch is trapped within the BSG matrix by a proteinaceous barrier.",
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Faulds, CB, Collins, S, Robertson, JA, Treimo, J, Eijsink, VGH, Hinz, SWA, Schols, HA, Buchert, J & Waldron, KW 2009, 'Protease-induced solubilisation of carbohydrates from brewers' spent grain', Journal of Cereal Science, vol. 50, no. 3, pp. 332-336. https://doi.org/10.1016/j.jcs.2009.01.004

Protease-induced solubilisation of carbohydrates from brewers' spent grain. / Faulds, Craig B.; Collins, Samuel; Robertson, James A.; Treimo, Janneke; Eijsink, Vincent G. H.; Hinz, Sandra W. A.; Schols, Henk A.; Buchert, Johanna; Waldron, Keith W.

In: Journal of Cereal Science, Vol. 50, No. 3, 2009, p. 332-336.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Protease-induced solubilisation of carbohydrates from brewers' spent grain

AU - Faulds, Craig B.

AU - Collins, Samuel

AU - Robertson, James A.

AU - Treimo, Janneke

AU - Eijsink, Vincent G. H.

AU - Hinz, Sandra W. A.

AU - Schols, Henk A.

AU - Buchert, Johanna

AU - Waldron, Keith W.

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N2 - The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose was derived from residual starch-derived products persisting in BSG after mashing. The proteases did not cleave the hydroxycinnamate ester linkages present on the arabinoxylan backbone, and thus do not behave as feruloyl esterases. However, the material solubilised from spent grain by the proteases contained up to 198 μg bound ferulic acid/g extract, which represented 8.6% of the total ferulic acid present in BSG. These results suggest that a portion of water-extractable feruloylated arabinoxylan and starch is trapped within the BSG matrix by a proteinaceous barrier.

AB - The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose was derived from residual starch-derived products persisting in BSG after mashing. The proteases did not cleave the hydroxycinnamate ester linkages present on the arabinoxylan backbone, and thus do not behave as feruloyl esterases. However, the material solubilised from spent grain by the proteases contained up to 198 μg bound ferulic acid/g extract, which represented 8.6% of the total ferulic acid present in BSG. These results suggest that a portion of water-extractable feruloylated arabinoxylan and starch is trapped within the BSG matrix by a proteinaceous barrier.

KW - brewers

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KW - starch

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Faulds CB, Collins S, Robertson JA, Treimo J, Eijsink VGH, Hinz SWA et al. Protease-induced solubilisation of carbohydrates from brewers' spent grain. Journal of Cereal Science. 2009;50(3):332-336. https://doi.org/10.1016/j.jcs.2009.01.004