Protease-induced solubilisation of carbohydrates from brewers' spent grain

Craig B. Faulds, Samuel Collins, James A. Robertson, Janneke Treimo, Vincent G. H. Eijsink, Sandra W. A. Hinz, Henk A. Schols, Johanna Buchert, Keith W. Waldron

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)


The impact of microbial proteases on the release of carbohydrates from BSG was studied. The proteases were able to release the non-cellulosic glucose, a portion of feruloylated arabinoxylan and over 50% of the protein from brewers' spent grain (BSG) after 24 h hydrolysis. The non-cellulosic glucose was derived from residual starch-derived products persisting in BSG after mashing. The proteases did not cleave the hydroxycinnamate ester linkages present on the arabinoxylan backbone, and thus do not behave as feruloyl esterases. However, the material solubilised from spent grain by the proteases contained up to 198 μg bound ferulic acid/g extract, which represented 8.6% of the total ferulic acid present in BSG. These results suggest that a portion of water-extractable feruloylated arabinoxylan and starch is trapped within the BSG matrix by a proteinaceous barrier.
Original languageEnglish
Pages (from-to)332-336
JournalJournal of Cereal Science
Issue number3
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed


  • brewers
  • spent grain
  • starch
  • arabinoxylan
  • protease
  • cereal by-products


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