Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno, Geza Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi, Tetsuya Haruyama (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.
Original languageEnglish
Pages (from-to)413 - 420
Number of pages8
JournalSensors and Materials
Volume16
Issue number8
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

immobilization
proteins
Proteins
Carrier Proteins
Molecules
Glass
Contacts (fluid mechanics)
molecules
Molecular weight
glass
low molecular weights
Adsorption
Substrates
chips
adsorption

Keywords

  • hydrophobins
  • protein array
  • high throughput
  • dot spot

Cite this

Ikeno, S., Szilvay, G., Linder, M., Aritomi, H., Ajimi, M., & Haruyama, T. (2004). Protein dot stamp using hydrophobin as immobilization carrier. Sensors and Materials, 16(8), 413 - 420.
Ikeno, Shinya ; Szilvay, Geza ; Linder, Markus ; Aritomi, Hijiri ; Ajimi, Masako ; Haruyama, Tetsuya. / Protein dot stamp using hydrophobin as immobilization carrier. In: Sensors and Materials. 2004 ; Vol. 16, No. 8. pp. 413 - 420.
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Ikeno, S, Szilvay, G, Linder, M, Aritomi, H, Ajimi, M & Haruyama, T 2004, 'Protein dot stamp using hydrophobin as immobilization carrier', Sensors and Materials, vol. 16, no. 8, pp. 413 - 420.

Protein dot stamp using hydrophobin as immobilization carrier. / Ikeno, Shinya; Szilvay, Geza; Linder, Markus; Aritomi, Hijiri; Ajimi, Masako; Haruyama, Tetsuya (Corresponding Author).

In: Sensors and Materials, Vol. 16, No. 8, 2004, p. 413 - 420.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Protein dot stamp using hydrophobin as immobilization carrier

AU - Ikeno, Shinya

AU - Szilvay, Geza

AU - Linder, Markus

AU - Aritomi, Hijiri

AU - Ajimi, Masako

AU - Haruyama, Tetsuya

PY - 2004

Y1 - 2004

N2 - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

AB - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

KW - hydrophobins

KW - protein array

KW - high throughput

KW - dot spot

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JO - Sensors and Materials

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Ikeno S, Szilvay G, Linder M, Aritomi H, Ajimi M, Haruyama T. Protein dot stamp using hydrophobin as immobilization carrier. Sensors and Materials. 2004;16(8):413 - 420.