Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno; Geza Szilvay; Markus Linder; Hijiri Aritomi; Masako Ajimi; Tetsuya Haruyama

Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno, Geza Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi, Tetsuya Haruyama (Corresponding Author)

BA3404 Enzyme and material biotechnology

Research output: Contribution to journal › Article › Scientific › peer-review

8 Citations (Scopus)

Abstract

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

Original language	English
Pages (from-to)	413 - 420
Number of pages	8
Journal	Sensors and Materials
Volume	16
Issue number	8
Publication status	Published - 2004
MoE publication type	A1 Journal article-refereed

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Keywords

hydrophobins
protein array
high throughput
dot spot

Cite this

Ikeno, S., Szilvay, G., Linder, M., Aritomi, H., Ajimi, M., & Haruyama, T. (2004). Protein dot stamp using hydrophobin as immobilization carrier. Sensors and Materials, 16(8), 413 - 420. https://myukk.org/SM2017/article.php?ss=10578

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T1 - Protein dot stamp using hydrophobin as immobilization carrier

AU - Ikeno, Shinya

AU - Szilvay, Geza

AU - Linder, Markus

AU - Aritomi, Hijiri

AU - Ajimi, Masako

AU - Haruyama, Tetsuya

PY - 2004

Y1 - 2004

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AB - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

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KW - protein array

KW - high throughput

KW - dot spot

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JF - Sensors and Materials

SN - 0914-4935

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https://myukk.org/SM2017/article.php?ss=10578