Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno; Geza Szilvay; Markus Linder; Hijiri Aritomi; Masako Ajimi; Tetsuya Haruyama

Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno, Geza Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi, Tetsuya Haruyama (Corresponding Author)

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

Original language	English
Pages (from-to)	413 - 420
Number of pages	8
Journal	Sensors and Materials
Volume	16
Issue number	8
Publication status	Published - 2004
MoE publication type	A1 Journal article-refereed

Keywords

hydrophobins
protein array
high throughput
dot spot

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title = "Protein dot stamp using hydrophobin as immobilization carrier",

abstract = "Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.",

keywords = "hydrophobins, protein array, high throughput, dot spot",

author = "Shinya Ikeno and Geza Szilvay and Markus Linder and Hijiri Aritomi and Masako Ajimi and Tetsuya Haruyama",

year = "2004",

language = "English",

volume = "16",

pages = "413 -- 420",

journal = "Sensors and Materials",

issn = "0914-4935",

publisher = "M Y U Scientific Publishing Division",

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TY - JOUR

T1 - Protein dot stamp using hydrophobin as immobilization carrier

AU - Ikeno, Shinya

AU - Szilvay, Geza

AU - Linder, Markus

AU - Aritomi, Hijiri

AU - Ajimi, Masako

AU - Haruyama, Tetsuya

PY - 2004

Y1 - 2004

N2 - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

AB - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.

KW - hydrophobins

KW - protein array

KW - high throughput

KW - dot spot

M3 - Article

VL - 16

SP - 413

EP - 420

JO - Sensors and Materials

JF - Sensors and Materials

SN - 0914-4935

IS - 8

ER -