Protein dot stamp using hydrophobin as immobilization carrier

Shinya Ikeno, Geza Szilvay, Markus Linder, Hijiri Aritomi, Masako Ajimi, Tetsuya Haruyama (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    8 Citations (Scopus)

    Abstract

    Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.
    Original languageEnglish
    Pages (from-to)413 - 420
    Number of pages8
    JournalSensors and Materials
    Volume16
    Issue number8
    Publication statusPublished - 2004
    MoE publication typeA1 Journal article-refereed

    Keywords

    • hydrophobins
    • protein array
    • high throughput
    • dot spot

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