Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 413 - 420 |
| Number of pages | 8 |
| Journal | Sensors and Materials |
| Volume | 16 |
| Issue number | 8 |
| Publication status | Published - 2004 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- hydrophobins
- protein array
- high throughput
- dot spot
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Protein dot stamp using hydrophobin as immobilization carrier. / Ikeno, Shinya; Szilvay, Geza; Linder, Markus; Aritomi, Hijiri; Ajimi, Masako; Haruyama, Tetsuya (Corresponding Author).
In: Sensors and Materials, Vol. 16, No. 8, 2004, p. 413 - 420.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Protein dot stamp using hydrophobin as immobilization carrier
AU - Ikeno, Shinya
AU - Szilvay, Geza
AU - Linder, Markus
AU - Aritomi, Hijiri
AU - Ajimi, Masako
AU - Haruyama, Tetsuya
PY - 2004
Y1 - 2004
N2 - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.
AB - Protein dot spot technology is presented using unique proteins such as hydrophobin HFB (from Trichoderma reesei). HFB is a protein of low molecular weight (7.8 kD) and is an outstanding hydrophobic molecule. The authors employ HFB as a carrier for protein dot spots. In this study, fluorescent-labeled HFB was spotted on a hydrophobic glass substrate using a solution drop contact spotter. HFB adsorbed on the glass surface uniformly. The results show that HFB provides prominent adsorption characteristics as a carrier protein. The small HFB molecule can be tagged onto other protein molecules through a conventional genetic procedure. By using HFB as a carrier protein, practical protein chip technology can be realized.
KW - hydrophobins
KW - protein array
KW - high throughput
KW - dot spot
M3 - Article
VL - 16
SP - 413
EP - 420
JO - Sensors and Materials
JF - Sensors and Materials
SN - 0914-4935
IS - 8
ER -