Protein engineering of surface loops: Preliminary X-ray analysis of the CHY155-165RHI mutant

Raj R.V. Dhanaraj, Jim E. Pitts, Phil Nugent, Ponsook Orprayoon, Jon B. Cooper, Tom L. Blundell, Jaana Uusitalo, Merja Penttila

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

The protein engineering programme at Birkbeck seeks to develop generic methods based on a design cycle involving biochemical preparation and characterization, determination and comparative analysis of three-dimensional structures, rule-based design, site-directed mutagenesis and expression of the mutants. Knowledge of the tertiary structure of proteins and the use of molecular modelling techniques provide a powerful approach for the design of novel biomolecules with specifically engineered properties. The aspartic proteinase family have been studied in detail by X-ray analysis and provide a suitable data base for the development of protein engineering design principles1–6. This family of endopeptidases belonging to a wide range of biological species with varying substrate specificities. The members of the family are of considerable commercial importance since enzymes such as chymosin and Mucor pusillus pepsin have been exploited by the food industry in cheese and soya processing, while renins, cathepsins and the retroviral proteinases are prime targets of the pharmaceutical industries in drug design.
Original languageEnglish
Title of host publicationAspartic Proteinases
Subtitle of host publicationStructure, Function, Biology, and Biomedical Implications
EditorsKenji Takahashi
Place of PublicationBoston
PublisherSpringer
Pages95-99
ISBN (Print)978-1-4613-5761-2
DOIs
Publication statusPublished - 1 Jan 1995
MoE publication typeA4 Article in a conference publication
Event5th International Conference on Aspartic Proteinases - Kawashima, Japan
Duration: 19 Sep 199324 Sep 1993
Conference number: 5

Publication series

SeriesAdvances in Experimental Medicine and Biology
Volume362
ISSN0065-2598

Conference

Conference5th International Conference on Aspartic Proteinases
CountryJapan
CityKawashima
Period19/09/9324/09/93

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