Protein engineering of surface loops: Preliminary X-ray analysis of the CHY155-165RHI mutant

Raj R.V. Dhanaraj, Jim E. Pitts, Phil Nugent, Ponsook Orprayoon, Jon B. Cooper, Tom L. Blundell, Jaana Uusitalo, Merja Penttila

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    Abstract

    The protein engineering programme at Birkbeck seeks to develop generic methods based on a design cycle involving biochemical preparation and characterization, determination and comparative analysis of three-dimensional structures, rule-based design, site-directed mutagenesis and expression of the mutants. Knowledge of the tertiary structure of proteins and the use of molecular modelling techniques provide a powerful approach for the design of novel biomolecules with specifically engineered properties. The aspartic proteinase family have been studied in detail by X-ray analysis and provide a suitable data base for the development of protein engineering design principles1–6. This family of endopeptidases belonging to a wide range of biological species with varying substrate specificities. The members of the family are of considerable commercial importance since enzymes such as chymosin and Mucor pusillus pepsin have been exploited by the food industry in cheese and soya processing, while renins, cathepsins and the retroviral proteinases are prime targets of the pharmaceutical industries in drug design.
    Original languageEnglish
    Title of host publicationAspartic Proteinases
    Subtitle of host publicationStructure, Function, Biology, and Biomedical Implications
    EditorsKenji Takahashi
    Place of PublicationBoston
    PublisherSpringer
    Pages95-99
    ISBN (Print)978-1-4613-5761-2
    DOIs
    Publication statusPublished - 1 Jan 1995
    MoE publication typeA4 Article in a conference publication
    Event5th International Conference on Aspartic Proteinases - Kawashima, Japan
    Duration: 19 Sep 199324 Sep 1993
    Conference number: 5

    Publication series

    SeriesAdvances in Experimental Medicine and Biology
    Volume362
    ISSN0065-2598

    Conference

    Conference5th International Conference on Aspartic Proteinases
    Country/TerritoryJapan
    CityKawashima
    Period19/09/9324/09/93

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