Protein production in filamentous fungal cells in the absence of inducing substrates

Michael Ward; Yun Luo; Felipe Oseas Bendezu; Mari Valkonen; Markku Saloheimo; Nina Aro; Tiina Pakula

Protein production in filamentous fungal cells in the absence of inducing substrates

Michael Ward (Inventor), Yun Luo (Inventor), Felipe Oseas Bendezu (Inventor), Mari Valkonen (Inventor), Markku Saloheimo (Inventor), Nina Aro (Inventor), Tiina Pakula (Inventor)

Danisco USA Inc.

Research output: Patent › Patent

Abstract

Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.

Patent family as of 26.8.2021
CN109790510 A 20190521 CN201780059992 20171003
EP3523415 A1 20190814 EP20170794124 20171003
JP2019528797 T2 20191017 JP20190539732T 20171003
KR20190057088 A 20190527 KR20197010929 20171003
US10876103 BB 20201229 US20170336500 20171003
US2019309276 AA 20191010 US20170336500 20171003
WO18067599 A1 20180412 WO2017US54983 20171003

Link to current patent family on right

Original language	English
Patent number	US2019309276
IPC	C12N 15/ 80 A I
Priority date	3/10/17
Publication status	Published - 10 Oct 2019
MoE publication type	H1 Granted patent

Cite this

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title = "Protein production in filamentous fungal cells in the absence of inducing substrates",

abstract = "Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Patent family as of 26.8.2021CN109790510 A 20190521 CN201780059992 20171003 EP3523415 A1 20190814 EP20170794124 20171003 JP2019528797 T2 20191017 JP20190539732T 20171003 KR20190057088 A 20190527 KR20197010929 20171003 US10876103 BB 20201229 US20170336500 20171003 US2019309276 AA 20191010 US20170336500 20171003 WO18067599 A1 20180412 WO2017US54983 20171003Link to current patent family on right",

author = "Michael Ward and Yun Luo and Bendezu, {Felipe Oseas} and Mari Valkonen and Markku Saloheimo and Nina Aro and Tiina Pakula",

year = "2019",

month = oct,

day = "10",

language = "English",

type = "Patent",

note = "US2019309276; C12N 15/ 80 A I",

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TY - PAT

T1 - Protein production in filamentous fungal cells in the absence of inducing substrates

AU - Ward, Michael

AU - Luo, Yun

AU - Bendezu, Felipe Oseas

AU - Valkonen, Mari

AU - Saloheimo, Markku

AU - Aro, Nina

AU - Pakula, Tiina

PY - 2019/10/10

Y1 - 2019/10/10

N2 - Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Patent family as of 26.8.2021CN109790510 A 20190521 CN201780059992 20171003 EP3523415 A1 20190814 EP20170794124 20171003 JP2019528797 T2 20191017 JP20190539732T 20171003 KR20190057088 A 20190527 KR20197010929 20171003 US10876103 BB 20201229 US20170336500 20171003 US2019309276 AA 20191010 US20170336500 20171003 WO18067599 A1 20180412 WO2017US54983 20171003Link to current patent family on right

AB - Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Patent family as of 26.8.2021CN109790510 A 20190521 CN201780059992 20171003 EP3523415 A1 20190814 EP20170794124 20171003 JP2019528797 T2 20191017 JP20190539732T 20171003 KR20190057088 A 20190527 KR20197010929 20171003 US10876103 BB 20201229 US20170336500 20171003 US2019309276 AA 20191010 US20170336500 20171003 WO18067599 A1 20180412 WO2017US54983 20171003Link to current patent family on right

UR - https://worldwide.espacenet.com/patent/search/family/060245185/publication/US10876103B2?q=US10876103

M3 - Patent

M1 - US2019309276

ER -

Protein production in filamentous fungal cells in the absence of inducing substrates

Abstract

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