Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae

Laura Salusjärvi, Matti Kankainen, Heini Koivistoinen, Merja Penttilä, Laura Ruohonen

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Economically feasible production of fuel ethanol from lignocellulosic material relies on quantitative conversion of the carbon present in the biomass that may contain 30-40% hemicellulose. Xylose is the most abundant pentose sugar in the hemicellulose and it is the second only to glucose in natural abundance. Hexose sugars are readily utilised by most industrial micro-organisms but efficient utilization of pentoses present in the hemicellulose fraction is still a challenge. Xylose fermentation by Saccharomyces cerevisiae has been enabled by introducing the genes encoding xylose reductase (XYL1, XR) and xylitol dehydrogenase (XYL2, XDH) from the yeast Pichia stipitis naturally utilising xylose. Over-expression of the endogenous xylulokinase-encoding gene (XKS1) of S. cerevisiae further enhances xylose consumption (1). Introduction of the xylose-utilisation pathway into S. cerevisiae not naturally fermenting pentose sugars has a major impact on the overall cellular metabolism as the carbon introduced will now flow through the pentose phosphate pathway. In addition, the introduction of redox enzymes into S. cerevisiae affects the redox balance of the cell as xylose reductase has a preference for NADPH, while xylitol dehydrogenase is specific for NAD+. This has been attributed to be one of the major reasons for inefficient incorporation of xylose-derived carbon into the central carbon pathways leading to ethanol by the oxido-reductive pathway. Genome wide approaches offer an attractive and global strategy to study the overall cellular metabolism under different physiological conditions. We have studied both on proteomic and genomic level and under different culture conditions the recombinant S. cerevisiae to reveal novel changes in the metabolism of xylose fermenting yeast (2, 3, 4).
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages157
ISBN (Electronic)978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

NameVTT Symposium
PublisherVTT
Number245
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

Fingerprint

proteome
xylose
Saccharomyces cerevisiae
transcription (genetics)
pentoses
hemicellulose
xylitol
carbon
sugars
metabolism
Scheffersomyces stipitis
ethanol fuels
yeasts
fuel production
NAD (coenzyme)
hexoses
NADP (coenzyme)
proteomics
genes
ethanol

Cite this

Salusjärvi, L., Kankainen, M., Koivistoinen, H., Penttilä, M., & Ruohonen, L. (2007). Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 157). [P99] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 245
Salusjärvi, Laura ; Kankainen, Matti ; Koivistoinen, Heini ; Penttilä, Merja ; Ruohonen, Laura. / Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae. 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 157 (VTT Symposium; No. 245).
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Salusjärvi, L, Kankainen, M, Koivistoinen, H, Penttilä, M & Ruohonen, L 2007, Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae. in 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3., P99, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 245, pp. 157, 3rd European Federation of Biotechnology Conference , Helsinki, Finland, 13/06/07.

Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae. / Salusjärvi, Laura; Kankainen, Matti; Koivistoinen, Heini; Penttilä, Merja; Ruohonen, Laura.

3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. p. 157 P99 (VTT Symposium; No. 245).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae

AU - Salusjärvi, Laura

AU - Kankainen, Matti

AU - Koivistoinen, Heini

AU - Penttilä, Merja

AU - Ruohonen, Laura

PY - 2007

Y1 - 2007

N2 - Economically feasible production of fuel ethanol from lignocellulosic material relies on quantitative conversion of the carbon present in the biomass that may contain 30-40% hemicellulose. Xylose is the most abundant pentose sugar in the hemicellulose and it is the second only to glucose in natural abundance. Hexose sugars are readily utilised by most industrial micro-organisms but efficient utilization of pentoses present in the hemicellulose fraction is still a challenge. Xylose fermentation by Saccharomyces cerevisiae has been enabled by introducing the genes encoding xylose reductase (XYL1, XR) and xylitol dehydrogenase (XYL2, XDH) from the yeast Pichia stipitis naturally utilising xylose. Over-expression of the endogenous xylulokinase-encoding gene (XKS1) of S. cerevisiae further enhances xylose consumption (1). Introduction of the xylose-utilisation pathway into S. cerevisiae not naturally fermenting pentose sugars has a major impact on the overall cellular metabolism as the carbon introduced will now flow through the pentose phosphate pathway. In addition, the introduction of redox enzymes into S. cerevisiae affects the redox balance of the cell as xylose reductase has a preference for NADPH, while xylitol dehydrogenase is specific for NAD+. This has been attributed to be one of the major reasons for inefficient incorporation of xylose-derived carbon into the central carbon pathways leading to ethanol by the oxido-reductive pathway. Genome wide approaches offer an attractive and global strategy to study the overall cellular metabolism under different physiological conditions. We have studied both on proteomic and genomic level and under different culture conditions the recombinant S. cerevisiae to reveal novel changes in the metabolism of xylose fermenting yeast (2, 3, 4).

AB - Economically feasible production of fuel ethanol from lignocellulosic material relies on quantitative conversion of the carbon present in the biomass that may contain 30-40% hemicellulose. Xylose is the most abundant pentose sugar in the hemicellulose and it is the second only to glucose in natural abundance. Hexose sugars are readily utilised by most industrial micro-organisms but efficient utilization of pentoses present in the hemicellulose fraction is still a challenge. Xylose fermentation by Saccharomyces cerevisiae has been enabled by introducing the genes encoding xylose reductase (XYL1, XR) and xylitol dehydrogenase (XYL2, XDH) from the yeast Pichia stipitis naturally utilising xylose. Over-expression of the endogenous xylulokinase-encoding gene (XKS1) of S. cerevisiae further enhances xylose consumption (1). Introduction of the xylose-utilisation pathway into S. cerevisiae not naturally fermenting pentose sugars has a major impact on the overall cellular metabolism as the carbon introduced will now flow through the pentose phosphate pathway. In addition, the introduction of redox enzymes into S. cerevisiae affects the redox balance of the cell as xylose reductase has a preference for NADPH, while xylitol dehydrogenase is specific for NAD+. This has been attributed to be one of the major reasons for inefficient incorporation of xylose-derived carbon into the central carbon pathways leading to ethanol by the oxido-reductive pathway. Genome wide approaches offer an attractive and global strategy to study the overall cellular metabolism under different physiological conditions. We have studied both on proteomic and genomic level and under different culture conditions the recombinant S. cerevisiae to reveal novel changes in the metabolism of xylose fermenting yeast (2, 3, 4).

M3 - Conference abstract in proceedings

SN - 978-951-38-6313-5

T3 - VTT Symposium

SP - 157

BT - 3rd European Federation of Biotechnology Conference

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Salusjärvi L, Kankainen M, Koivistoinen H, Penttilä M, Ruohonen L. Proteome and transcription analysis of recombinant xylose-utilising Saccharomyces cerevisiae. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo: VTT Technical Research Centre of Finland. 2007. p. 157. P99. (VTT Symposium; No. 245).