Pulmonary inflammation and tissue damage in the mouse lung after exposure to PM samples from biomass heating appliances of old and modern technologies

Mikko S. Happo (Corresponding Author), Oskari Uski, Pasi I. Jalava, Joachim Kelz, Thomas Brunner, Pasi Hakulinen, Jorma Mäki-Paakkanen, Veli-Matti Kosma, Jorma Jokiniemi, Ingwald Obernberger, Maija-Riitta Hirvonen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Current levels of ambient air fine particulate matter (PM2.5) are associated with mortality and morbidity in urban populations worldwide. In residential areas wood combustion is one of the main sources of PM2.5 emissions, especially during wintertime. However, the adverse health effects of particulate emissions from the modern heating appliances and fuels are poorly known. In this study, health related toxicological properties of PM1 emissions from five modern and two old technology appliances were examined. The PM1 samples were collected by using a Dekati® Gravimetric Impactor (DGI). The collected samples were weighed and extracted with methanol for chemical and toxicological analyses. Healthy C57BL/6J mice were intratracheally exposed to a single dose of 1, 3, 10 or 15 mg/kg of the particulate samples for 4, 18 or 24 h. Thereafter, the lungs were lavaged and bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation, cytotoxicity and genotoxicity. Lungs of 24 h exposed mice were collected for inspection of pulmonary tissue damage. There were substantial differences in the combustion qualities of old and modern technology appliances. Modern technology appliances had the lowest PM1 (mg/MJ) emissions, but they induced the highest inflammatory, cytotoxic and genotoxic activities. In contrast, old technology appliances had clearly the highest PM1 (mg/MJ) emissions, but their effect in the mouse lungs were the lowest. Increased inflammatory activity was associated with ash related components of the emissions, whereas high PAH concentrations were correlating with the smallest detected responses, possibly due to their immunosuppressive effect.
Original languageEnglish
Pages (from-to)256-266
JournalScience of the Total Environment
Volume443
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Biomass
Tissue
heating
Heating
damage
biomass
Ashes
Health
Particulate emissions
Particulate Matter
Immunosuppressive Agents
Cytotoxicity
Polycyclic aromatic hydrocarbons
combustion
Methanol
Wood
Inspection
genotoxicity
urban population
morbidity

Keywords

  • Chemical composition
  • cytotoxicity
  • genotoxicity
  • inflammation
  • particulate matter
  • small-scale wood combustion

Cite this

Happo, Mikko S. ; Uski, Oskari ; Jalava, Pasi I. ; Kelz, Joachim ; Brunner, Thomas ; Hakulinen, Pasi ; Mäki-Paakkanen, Jorma ; Kosma, Veli-Matti ; Jokiniemi, Jorma ; Obernberger, Ingwald ; Hirvonen, Maija-Riitta. / Pulmonary inflammation and tissue damage in the mouse lung after exposure to PM samples from biomass heating appliances of old and modern technologies. In: Science of the Total Environment. 2013 ; Vol. 443. pp. 256-266.
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abstract = "Current levels of ambient air fine particulate matter (PM2.5) are associated with mortality and morbidity in urban populations worldwide. In residential areas wood combustion is one of the main sources of PM2.5 emissions, especially during wintertime. However, the adverse health effects of particulate emissions from the modern heating appliances and fuels are poorly known. In this study, health related toxicological properties of PM1 emissions from five modern and two old technology appliances were examined. The PM1 samples were collected by using a Dekati{\circledR} Gravimetric Impactor (DGI). The collected samples were weighed and extracted with methanol for chemical and toxicological analyses. Healthy C57BL/6J mice were intratracheally exposed to a single dose of 1, 3, 10 or 15 mg/kg of the particulate samples for 4, 18 or 24 h. Thereafter, the lungs were lavaged and bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation, cytotoxicity and genotoxicity. Lungs of 24 h exposed mice were collected for inspection of pulmonary tissue damage. There were substantial differences in the combustion qualities of old and modern technology appliances. Modern technology appliances had the lowest PM1 (mg/MJ) emissions, but they induced the highest inflammatory, cytotoxic and genotoxic activities. In contrast, old technology appliances had clearly the highest PM1 (mg/MJ) emissions, but their effect in the mouse lungs were the lowest. Increased inflammatory activity was associated with ash related components of the emissions, whereas high PAH concentrations were correlating with the smallest detected responses, possibly due to their immunosuppressive effect.",
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author = "Happo, {Mikko S.} and Oskari Uski and Jalava, {Pasi I.} and Joachim Kelz and Thomas Brunner and Pasi Hakulinen and Jorma M{\"a}ki-Paakkanen and Veli-Matti Kosma and Jorma Jokiniemi and Ingwald Obernberger and Maija-Riitta Hirvonen",
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Happo, MS, Uski, O, Jalava, PI, Kelz, J, Brunner, T, Hakulinen, P, Mäki-Paakkanen, J, Kosma, V-M, Jokiniemi, J, Obernberger, I & Hirvonen, M-R 2013, 'Pulmonary inflammation and tissue damage in the mouse lung after exposure to PM samples from biomass heating appliances of old and modern technologies', Science of the Total Environment, vol. 443, pp. 256-266. https://doi.org/10.1016/j.scitotenv.2012.11.004

Pulmonary inflammation and tissue damage in the mouse lung after exposure to PM samples from biomass heating appliances of old and modern technologies. / Happo, Mikko S. (Corresponding Author); Uski, Oskari; Jalava, Pasi I.; Kelz, Joachim; Brunner, Thomas; Hakulinen, Pasi; Mäki-Paakkanen, Jorma; Kosma, Veli-Matti; Jokiniemi, Jorma; Obernberger, Ingwald; Hirvonen, Maija-Riitta.

In: Science of the Total Environment, Vol. 443, 2013, p. 256-266.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Pulmonary inflammation and tissue damage in the mouse lung after exposure to PM samples from biomass heating appliances of old and modern technologies

AU - Happo, Mikko S.

AU - Uski, Oskari

AU - Jalava, Pasi I.

AU - Kelz, Joachim

AU - Brunner, Thomas

AU - Hakulinen, Pasi

AU - Mäki-Paakkanen, Jorma

AU - Kosma, Veli-Matti

AU - Jokiniemi, Jorma

AU - Obernberger, Ingwald

AU - Hirvonen, Maija-Riitta

PY - 2013

Y1 - 2013

N2 - Current levels of ambient air fine particulate matter (PM2.5) are associated with mortality and morbidity in urban populations worldwide. In residential areas wood combustion is one of the main sources of PM2.5 emissions, especially during wintertime. However, the adverse health effects of particulate emissions from the modern heating appliances and fuels are poorly known. In this study, health related toxicological properties of PM1 emissions from five modern and two old technology appliances were examined. The PM1 samples were collected by using a Dekati® Gravimetric Impactor (DGI). The collected samples were weighed and extracted with methanol for chemical and toxicological analyses. Healthy C57BL/6J mice were intratracheally exposed to a single dose of 1, 3, 10 or 15 mg/kg of the particulate samples for 4, 18 or 24 h. Thereafter, the lungs were lavaged and bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation, cytotoxicity and genotoxicity. Lungs of 24 h exposed mice were collected for inspection of pulmonary tissue damage. There were substantial differences in the combustion qualities of old and modern technology appliances. Modern technology appliances had the lowest PM1 (mg/MJ) emissions, but they induced the highest inflammatory, cytotoxic and genotoxic activities. In contrast, old technology appliances had clearly the highest PM1 (mg/MJ) emissions, but their effect in the mouse lungs were the lowest. Increased inflammatory activity was associated with ash related components of the emissions, whereas high PAH concentrations were correlating with the smallest detected responses, possibly due to their immunosuppressive effect.

AB - Current levels of ambient air fine particulate matter (PM2.5) are associated with mortality and morbidity in urban populations worldwide. In residential areas wood combustion is one of the main sources of PM2.5 emissions, especially during wintertime. However, the adverse health effects of particulate emissions from the modern heating appliances and fuels are poorly known. In this study, health related toxicological properties of PM1 emissions from five modern and two old technology appliances were examined. The PM1 samples were collected by using a Dekati® Gravimetric Impactor (DGI). The collected samples were weighed and extracted with methanol for chemical and toxicological analyses. Healthy C57BL/6J mice were intratracheally exposed to a single dose of 1, 3, 10 or 15 mg/kg of the particulate samples for 4, 18 or 24 h. Thereafter, the lungs were lavaged and bronchoalveolar lavage fluid (BALF) was assayed for indicators of inflammation, cytotoxicity and genotoxicity. Lungs of 24 h exposed mice were collected for inspection of pulmonary tissue damage. There were substantial differences in the combustion qualities of old and modern technology appliances. Modern technology appliances had the lowest PM1 (mg/MJ) emissions, but they induced the highest inflammatory, cytotoxic and genotoxic activities. In contrast, old technology appliances had clearly the highest PM1 (mg/MJ) emissions, but their effect in the mouse lungs were the lowest. Increased inflammatory activity was associated with ash related components of the emissions, whereas high PAH concentrations were correlating with the smallest detected responses, possibly due to their immunosuppressive effect.

KW - Chemical composition

KW - cytotoxicity

KW - genotoxicity

KW - inflammation

KW - particulate matter

KW - small-scale wood combustion

U2 - 10.1016/j.scitotenv.2012.11.004

DO - 10.1016/j.scitotenv.2012.11.004

M3 - Article

VL - 443

SP - 256

EP - 266

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

ER -