Purification and characterization of a xylanase from Bipolaris sorokiniana

Sari Peltonen, Reijo Karjalainen, Marja-Leena Niku-Paavola

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Bipolaris sorokiniana, a cereal fungal pathogen, showed xylanase activity when grown on minimal salt medium containing 2% of cell wall prepared from barley leaves as the sole carbon source. The most abundant of the xylanases, 1,4-β-D-xylan xylanohydrolase (EC 3.2.1.8), was purified to homogeneity by S-Sepharose chromatography, and its properties were determined. The induction of xylanase was highest on the 12th day after inoculation in shake flask culture. The purified xylanase had a molecular weight of 30 kDa and an isoelectric point of 9·5. The pH and temperature optima of the B. sorokiniana xylanase activity were 5·5 and 70°C respectively. The enzyme was stable over the pH range 5–10 but the stability towards temperature fell sharply above 55°.
Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalMycological Research
Volume98
Issue number1
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

Fingerprint

Bipolaris sorokiniana
xylanases
purification
Endo-1,4-beta Xylanases
Agarose Chromatography
Batch Cell Culture Techniques
Temperature
Isoelectric Point
Hordeum
barley
cereal
Cell Wall
homogeneity
inoculation
chromatography
Carbon
pathogen
Salts
Molecular Weight
temperature

Cite this

Peltonen, Sari ; Karjalainen, Reijo ; Niku-Paavola, Marja-Leena. / Purification and characterization of a xylanase from Bipolaris sorokiniana. In: Mycological Research. 1994 ; Vol. 98, No. 1. pp. 67-73.
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Purification and characterization of a xylanase from Bipolaris sorokiniana. / Peltonen, Sari; Karjalainen, Reijo; Niku-Paavola, Marja-Leena.

In: Mycological Research, Vol. 98, No. 1, 1994, p. 67-73.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Purification and characterization of a xylanase from Bipolaris sorokiniana

AU - Peltonen, Sari

AU - Karjalainen, Reijo

AU - Niku-Paavola, Marja-Leena

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PY - 1994

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AB - Bipolaris sorokiniana, a cereal fungal pathogen, showed xylanase activity when grown on minimal salt medium containing 2% of cell wall prepared from barley leaves as the sole carbon source. The most abundant of the xylanases, 1,4-β-D-xylan xylanohydrolase (EC 3.2.1.8), was purified to homogeneity by S-Sepharose chromatography, and its properties were determined. The induction of xylanase was highest on the 12th day after inoculation in shake flask culture. The purified xylanase had a molecular weight of 30 kDa and an isoelectric point of 9·5. The pH and temperature optima of the B. sorokiniana xylanase activity were 5·5 and 70°C respectively. The enzyme was stable over the pH range 5–10 but the stability towards temperature fell sharply above 55°.

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