Purification and characterization of two β-mannanases from Trichoderma reesei

Henrik Stålbrand, Matti Siika-aho (Corresponding Author), Maija Tenkanen, Liisa Viikari

    Research output: Contribution to journalArticleScientificpeer-review

    195 Citations (Scopus)

    Abstract

    Five enzymes with mannanase activity were separated from Trichoderma reesei culture filtrate using analytical isoelectric focusing and subsequently detected with the zymogram technique. The crude enzymes had isoelectric points in the range of 3.6–6.5. Two of the mannanases with pI values of 4.6 and 5.4 were purified using ion-exchange chromatography, affinity chromatography and chromatofocusing. The molecular weights determined with SDS-PAGE were 51 000 (mannanase pI 4.6) and 53 000 (mannanase pI 5.4). The two enzymes had similar properties with respect to pH optimae and pH stabilities. Both mannanases hydrolyzed ivory nut mannan mainly to mannotriose and mannobiose. The specific activities (against locust bean gum) of the purified enzymes were 1860 and 1430 nkat mg−1 for the pI 4.6 and pI 5.4 mannanases, respectively.

    Original languageEnglish
    Pages (from-to)229 - 242
    Number of pages14
    JournalJournal of Biotechnology
    Volume29
    Issue number3
    DOIs
    Publication statusPublished - 1993
    MoE publication typeA1 Journal article-refereed

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