TY - JOUR
T1 - Purification, crystallization and preliminary X-ray diffraction analysis of the Trichoderma reesei hydrophobin HFBI
AU - Askolin, Sanna
AU - Turkenburg, Johan P.
AU - Tenkanen, Maija
AU - Uotila, Sinikka
AU - Wilson, Keith S.
AU - Penttilä, Merja
AU - Visuri, Kalevi
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Hydrophobins are fungal proteins that are capable of altering the hydrophobicity of surfaces by self-assembly at hydrophilic-hydrophobic interfaces. Here, the growth of hydrophobin crystals suitable for X-ray crystallography is reported. The hydrophobin HFBI from Trichoderma reesei was crystallized by vapour diffusion in hanging drops in 30% PEG 4000, 0.1 M sodium citrate pH 4.3 buffer containing 0.2 M ammonium acetate and CYMAL-5 detergent (initial concentration of 2.4 mM). HFBI crystals are hexagonal and belong to space group P61 (or P65), with unit-cell parameters a = b = 45.9, c = 307.2 Å. The HFBI used in the crystallization experiments was purified from fungal cell walls.
AB - Hydrophobins are fungal proteins that are capable of altering the hydrophobicity of surfaces by self-assembly at hydrophilic-hydrophobic interfaces. Here, the growth of hydrophobin crystals suitable for X-ray crystallography is reported. The hydrophobin HFBI from Trichoderma reesei was crystallized by vapour diffusion in hanging drops in 30% PEG 4000, 0.1 M sodium citrate pH 4.3 buffer containing 0.2 M ammonium acetate and CYMAL-5 detergent (initial concentration of 2.4 mM). HFBI crystals are hexagonal and belong to space group P61 (or P65), with unit-cell parameters a = b = 45.9, c = 307.2 Å. The HFBI used in the crystallization experiments was purified from fungal cell walls.
KW - hydrophobins
KW - HFBI
UR - http://www.scopus.com/inward/record.url?scp=16644366964&partnerID=8YFLogxK
U2 - 10.1107/S0907444904019754
DO - 10.1107/S0907444904019754
M3 - Article
C2 - 15388947
AN - SCOPUS:16644366964
SN - 0907-4449
VL - 60
SP - 1903
EP - 1905
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 10
ER -