Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

Martti Alkio, Christina Conzalez, Matti Jäntti, Olli Aaltonen

Research output: Contribution to journalArticleScientificpeer-review

59 Citations (Scopus)

Abstract

The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

Original languageEnglish
Pages (from-to)315 - 321
Number of pages7
JournalJournal of the American Oil Chemists' Society
Volume77
Issue number3
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Polyunsaturated fatty acids
Docosahexaenoic Acids
Unsaturated Fatty Acids
Purification
Esters
Oils
Acids
Eicosapentaenoic Acid
Silanes
Operating costs
Carbon Dioxide
Silicon Dioxide
Supercritical Fluid Chromatography
Economics
Carbon dioxide
Silica

Cite this

Alkio, Martti ; Conzalez, Christina ; Jäntti, Matti ; Aaltonen, Olli. / Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography. In: Journal of the American Oil Chemists' Society. 2000 ; Vol. 77, No. 3. pp. 315 - 321.
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abstract = "The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt{\%} purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt{\%}. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.",
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Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography. / Alkio, Martti; Conzalez, Christina; Jäntti, Matti; Aaltonen, Olli.

In: Journal of the American Oil Chemists' Society, Vol. 77, No. 3, 2000, p. 315 - 321.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

AU - Alkio, Martti

AU - Conzalez, Christina

AU - Jäntti, Matti

AU - Aaltonen, Olli

PY - 2000

Y1 - 2000

N2 - The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

AB - The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

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VL - 77

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EP - 321

JO - Journal of the American Oil Chemists' Society

JF - Journal of the American Oil Chemists' Society

SN - 0003-021X

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