Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

Martti Alkio; Christina Conzalez; Matti Jäntti; Olli Aaltonen

doi:10.1007/s11746-000-0051-3

Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

Martti Alkio, Christina Conzalez, Matti Jäntti, Olli Aaltonen

VTT Technical Research Centre of Finland

GAIKER Technology Centre

Research output: Contribution to journal › Article › Scientific › peer-review

70 Citations (Scopus)

Abstract

The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO₂ as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

Original language	English
Pages (from-to)	315-321
Journal	Journal of the American Oil Chemists' Society
Volume	77
Issue number	3
DOIs	https://doi.org/10.1007/s11746-000-0051-3
Publication status	Published - 2000
MoE publication type	A1 Journal article-refereed

Access to Document

10.1007/s11746-000-0051-3

Cite this

@article{e4a03b6b529940f6ba7ce21dd02daccb,

title = "Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography",

abstract = "The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.",

author = "Martti Alkio and Christina Conzalez and Matti J{\"a}ntti and Olli Aaltonen",

year = "2000",

doi = "10.1007/s11746-000-0051-3",

language = "English",

volume = "77",

pages = "315--321",

journal = "Journal of the American Oil Chemists' Society",

issn = "0003-021X",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

AU - Alkio, Martti

AU - Conzalez, Christina

AU - Jäntti, Matti

AU - Aaltonen, Olli

PY - 2000

Y1 - 2000

N2 - The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

AB - The technical and economic feasibility of producing docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-ethyl ester concentrates from transesterified tuna oil using supercritical fluid chromatography (SFC) was studied. A systematic experimental procedure was used to find the optimal values for process parameters and the maximal production rate. DHA ester concentrates up to 95 wt% purity were obtained in one chromatographic step with SFC, using CO2 as the mobile phase at 65°C and 145 bar and octadecyl silane-type reversed-phase silica as the stationary phase. DHA ester, 0.85 g/(kg stationary phase · h) and 0.23 g EPA ester/(kg stationary phase · h) can be simutaneously produced at the respective purities of 90 and 50 wt%. The process for producing 1,000 kg DHA concentrate and 410 kg EPA concentrate per year requires 160 kg stationary phase and 2.6 tons/h carbon dioxide eluant recycle. The SFC operating cost is U.S. $550/kg DHA and EPA ethyl ester concentrate.

U2 - 10.1007/s11746-000-0051-3

DO - 10.1007/s11746-000-0051-3

M3 - Article

SN - 0003-021X

VL - 77

SP - 315

EP - 321

JO - Journal of the American Oil Chemists' Society

JF - Journal of the American Oil Chemists' Society

IS - 3

ER -

Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography

Abstract

Access to Document

Fingerprint

Cite this