Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats

Whyara Karoline Almeida da Costa, Larissa Ramalho Brandão, Maria Elena Martino, Estefânia Fernandes Garcia, Adriano Francisco Alves, Evandro Leite de Souza, Jailane de Souza Aquino, Maria Saarela, François Leulier, Hubert Vidal, Marciane Magnani (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

Tropical fruit and their industrial processing byproducts have been considered sources of probiotic Lactobacillus. Sixteen tropical fruit-derived Lactobacillus strains were assessed for growth-promoting effects using a host-commensal nutrient scarcity model with Drosophila melanogaster (Dm). Two Lactobacillus strains (L. plantarum 49 and L. plantarum 201) presenting the most significant effects (p ≤ .005) on Dm growth were selected and evaluated for their safety and beneficial effects in adult male Wistar rats during 28 days of administration of 9 log CFU/day, followed by 14 days of wash-out. Daily administration of L. plantarum 49 and L. plantarum 201 did not affect (p > .05) food intake or morphometric parameters. Both strains were associated with reduction (p ≤ .05) in blood glucose levels after 28 days of administration and after wash-out period; glucose levels remained reduced only in the group that received L. plantarum 49. Both strains were able to reduce (p ≤ .05) total cholesterol levels after 14 days of administration; after the wash-out period these levels remained reduced only in the group that received L. plantarum 201. L. plantarum 49 and L. plantarum 201 were detected in the intestine and did not cause alteration or translocate to spleen, kidneys or liver during the experimental or wash-out period. These results indicate that L. plantarum 49 and L. plantarum 201 present potential for use as probiotics with intrinsic abilities to modulate biochemical parameters of interest for the management of metabolic diseases.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalFood Research International
Volume124
DOIs
Publication statusPublished - 1 Oct 2019
MoE publication typeNot Eligible

Fingerprint

Lactobacillus plantarum
tropical and subtropical fruits
Probiotics
probiotics
blood glucose
Blood Glucose
Wistar Rats
Fruit
Cholesterol
cholesterol
rats
Lactobacillus
Drosophila melanogaster
Metabolic Diseases
metabolic diseases
Growth
processing technology
byproducts
Intestines
food intake

Keywords

  • Grow promotion
  • Lactobacillus plantarum
  • Metabolic modulation
  • Probiotics
  • Safety

Cite this

da Costa, Whyara Karoline Almeida ; Brandão, Larissa Ramalho ; Martino, Maria Elena ; Garcia, Estefânia Fernandes ; Alves, Adriano Francisco ; de Souza, Evandro Leite ; de Souza Aquino, Jailane ; Saarela, Maria ; Leulier, François ; Vidal, Hubert ; Magnani, Marciane. / Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats. In: Food Research International. 2019 ; Vol. 124. pp. 109-117.
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abstract = "Tropical fruit and their industrial processing byproducts have been considered sources of probiotic Lactobacillus. Sixteen tropical fruit-derived Lactobacillus strains were assessed for growth-promoting effects using a host-commensal nutrient scarcity model with Drosophila melanogaster (Dm). Two Lactobacillus strains (L. plantarum 49 and L. plantarum 201) presenting the most significant effects (p ≤ .005) on Dm growth were selected and evaluated for their safety and beneficial effects in adult male Wistar rats during 28 days of administration of 9 log CFU/day, followed by 14 days of wash-out. Daily administration of L. plantarum 49 and L. plantarum 201 did not affect (p > .05) food intake or morphometric parameters. Both strains were associated with reduction (p ≤ .05) in blood glucose levels after 28 days of administration and after wash-out period; glucose levels remained reduced only in the group that received L. plantarum 49. Both strains were able to reduce (p ≤ .05) total cholesterol levels after 14 days of administration; after the wash-out period these levels remained reduced only in the group that received L. plantarum 201. L. plantarum 49 and L. plantarum 201 were detected in the intestine and did not cause alteration or translocate to spleen, kidneys or liver during the experimental or wash-out period. These results indicate that L. plantarum 49 and L. plantarum 201 present potential for use as probiotics with intrinsic abilities to modulate biochemical parameters of interest for the management of metabolic diseases.",
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da Costa, WKA, Brandão, LR, Martino, ME, Garcia, EF, Alves, AF, de Souza, EL, de Souza Aquino, J, Saarela, M, Leulier, F, Vidal, H & Magnani, M 2019, 'Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats', Food Research International, vol. 124, pp. 109-117. https://doi.org/10.1016/j.foodres.2018.08.035

Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats. / da Costa, Whyara Karoline Almeida; Brandão, Larissa Ramalho; Martino, Maria Elena; Garcia, Estefânia Fernandes; Alves, Adriano Francisco; de Souza, Evandro Leite; de Souza Aquino, Jailane; Saarela, Maria; Leulier, François; Vidal, Hubert; Magnani, Marciane (Corresponding Author).

In: Food Research International, Vol. 124, 01.10.2019, p. 109-117.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats

AU - da Costa, Whyara Karoline Almeida

AU - Brandão, Larissa Ramalho

AU - Martino, Maria Elena

AU - Garcia, Estefânia Fernandes

AU - Alves, Adriano Francisco

AU - de Souza, Evandro Leite

AU - de Souza Aquino, Jailane

AU - Saarela, Maria

AU - Leulier, François

AU - Vidal, Hubert

AU - Magnani, Marciane

PY - 2019/10/1

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