Abstract
The Raman spectra of the samples were measured with a CDD‐Raman spectrometer. Two Raman quantification methods were used: (1) relative peak heights of characteristic signals of the amphetamine and the internal standard; and (2) multivariate calibration by partial least squares (PLS) based on second derivative of the spectra.
For the determination of the peak height ratio, the spectra were baseline corrected and the peak height ratio (hamphetamine at 994 cm−1/hinternal standard at 880 cm−1) was calculated. For the PLS analysis, the wave number interval of 1300–630 cm−1 (348 data points) was chosen.
No manual baseline correction was performed, but the spectra were differentiated twice to obtain their second derivatives, which were further analyzed. The Raman results were well in line with validated reference LC results when the Raman samples were analyzed within 2 h after dissolution.
The present results clearly show that Raman spectroscopy is a good tool for rapid (acquisition time 1 min) and accurate quantitative analysis of street samples that contain illicit drugs and unknown adulterants and impurities
| Original language | English |
|---|---|
| Pages (from-to) | 88-92 |
| Journal | Journal of Forensic Sciences |
| Volume | 52 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 |
| MoE publication type | A1 Journal article-refereed |
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Quantification of the amphetamine content in seized street samples by raman spectroscopy. / Katainen, Erja; Elomaa, Matti; Laakkonen, Ulla-Maija; Sippola, Erkki; Niemelä, Pentti; Suhonen, Janne; Järvinen, Kristiina.
In: Journal of Forensic Sciences, Vol. 52, No. 1, 2007, p. 88-92.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Quantification of the amphetamine content in seized street samples by raman spectroscopy
AU - Katainen, Erja
AU - Elomaa, Matti
AU - Laakkonen, Ulla-Maija
AU - Sippola, Erkki
AU - Niemelä, Pentti
AU - Suhonen, Janne
AU - Järvinen, Kristiina
PY - 2007
Y1 - 2007
N2 - A Raman spectroscopy method for determining the drug content of street samples of amphetamine was developed by dissolving samples in an acidic solution containing an internal standard (sodium dihydrogen phosphate). The Raman spectra of the samples were measured with a CDD‐Raman spectrometer. Two Raman quantification methods were used: (1) relative peak heights of characteristic signals of the amphetamine and the internal standard; and (2) multivariate calibration by partial least squares (PLS) based on second derivative of the spectra. For the determination of the peak height ratio, the spectra were baseline corrected and the peak height ratio (hamphetamine at 994 cm−1/hinternal standard at 880 cm−1) was calculated. For the PLS analysis, the wave number interval of 1300–630 cm−1 (348 data points) was chosen. No manual baseline correction was performed, but the spectra were differentiated twice to obtain their second derivatives, which were further analyzed. The Raman results were well in line with validated reference LC results when the Raman samples were analyzed within 2 h after dissolution. The present results clearly show that Raman spectroscopy is a good tool for rapid (acquisition time 1 min) and accurate quantitative analysis of street samples that contain illicit drugs and unknown adulterants and impurities
AB - A Raman spectroscopy method for determining the drug content of street samples of amphetamine was developed by dissolving samples in an acidic solution containing an internal standard (sodium dihydrogen phosphate). The Raman spectra of the samples were measured with a CDD‐Raman spectrometer. Two Raman quantification methods were used: (1) relative peak heights of characteristic signals of the amphetamine and the internal standard; and (2) multivariate calibration by partial least squares (PLS) based on second derivative of the spectra. For the determination of the peak height ratio, the spectra were baseline corrected and the peak height ratio (hamphetamine at 994 cm−1/hinternal standard at 880 cm−1) was calculated. For the PLS analysis, the wave number interval of 1300–630 cm−1 (348 data points) was chosen. No manual baseline correction was performed, but the spectra were differentiated twice to obtain their second derivatives, which were further analyzed. The Raman results were well in line with validated reference LC results when the Raman samples were analyzed within 2 h after dissolution. The present results clearly show that Raman spectroscopy is a good tool for rapid (acquisition time 1 min) and accurate quantitative analysis of street samples that contain illicit drugs and unknown adulterants and impurities
U2 - 10.1111/j.1556-4029.2006.00306.x
DO - 10.1111/j.1556-4029.2006.00306.x
M3 - Article
VL - 52
SP - 88
EP - 92
JO - Journal of Forensic Sciences
JF - Journal of Forensic Sciences
SN - 0022-1198
IS - 1
ER -