Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 907 - 911 |
| Number of pages | 5 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 37 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Raman spectroscopy
- quantification
- amorphous content
- lactose
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Quantitative analysis of amorphous content of lactose using CCD-Raman spectroscopy. / Niemelä, Pentti (Corresponding Author); Päällysaho, Maarit; Harjunen, Päivi; Koivisto, Mikko; Lehto, Vesa-Pekka; Suhonen, Janne; Järvinen, Kristiina.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 37, No. 5, 2005, p. 907 - 911.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Quantitative analysis of amorphous content of lactose using CCD-Raman spectroscopy
AU - Niemelä, Pentti
AU - Päällysaho, Maarit
AU - Harjunen, Päivi
AU - Koivisto, Mikko
AU - Lehto, Vesa-Pekka
AU - Suhonen, Janne
AU - Järvinen, Kristiina
PY - 2005
Y1 - 2005
N2 - A Raman spectroscopy method was developed for the quantification of the amorphous content of lactose. Both physical mixtures and spray-dried samples were used and the results were compared with the IMC determinations. Sample inhomogeneities were averaged out by collecting multiple spectra from each sample, and the total measurement time remained below 10 min due to the high sensitivity of the CCD-Raman spectrometer used in the measurements. The obtained calibration error (SEC) for the physical mixtures was 1.3% (w/w) in the 0–100% amorphous content range and was reduced to 0.2% (w/w) in the 0–10% range of more practical interest. The crystallization heat values of the spray-dried samples showed a linear correlation with the Raman quantifications in the amorphous content range of 0–80%, but saturated over the 80% concentration. This finding suggests a reference value of ca. 60 J/g for the spray-dried samples, instead of the crystallization heat of amorphous lactose (ca. 50 J/g) valid in the IMC determinations of physical mixtures.
AB - A Raman spectroscopy method was developed for the quantification of the amorphous content of lactose. Both physical mixtures and spray-dried samples were used and the results were compared with the IMC determinations. Sample inhomogeneities were averaged out by collecting multiple spectra from each sample, and the total measurement time remained below 10 min due to the high sensitivity of the CCD-Raman spectrometer used in the measurements. The obtained calibration error (SEC) for the physical mixtures was 1.3% (w/w) in the 0–100% amorphous content range and was reduced to 0.2% (w/w) in the 0–10% range of more practical interest. The crystallization heat values of the spray-dried samples showed a linear correlation with the Raman quantifications in the amorphous content range of 0–80%, but saturated over the 80% concentration. This finding suggests a reference value of ca. 60 J/g for the spray-dried samples, instead of the crystallization heat of amorphous lactose (ca. 50 J/g) valid in the IMC determinations of physical mixtures.
KW - Raman spectroscopy
KW - quantification
KW - amorphous content
KW - lactose
U2 - 10.1016/j.jpba.2004.09.027
DO - 10.1016/j.jpba.2004.09.027
M3 - Article
VL - 37
SP - 907
EP - 911
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
IS - 5
ER -