Quantitative determination of wood-derived soluble oligosaccharides by HPLC

Johanna Buchert, Matti Siika-aho, Michael Bailey, Jurgen Puls, Anne Valkeajärvi, Jaakko Pere, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)


Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.
Original languageEnglish
Pages (from-to)785 - 790
Number of pages6
JournalBiotechnology Techniques
Issue number11
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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