Quantitative determination of wood-derived soluble oligosaccharides by HPLC

Johanna Buchert, Matti Siika-aho, Michael Bailey, Jurgen Puls, Anne Valkeajärvi, Jaakko Pere, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.
Original languageEnglish
Pages (from-to)785 - 790
Number of pages6
JournalBiotechnology Techniques
Volume7
Issue number11
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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Enzymatic hydrolysis
Oligosaccharides
Wood
Hydrolysis
High Pressure Liquid Chromatography
Monosaccharides
Sugars
Pulp
Carbon
Carbohydrates
Enzymes

Cite this

Buchert, Johanna ; Siika-aho, Matti ; Bailey, Michael ; Puls, Jurgen ; Valkeajärvi, Anne ; Pere, Jaakko ; Viikari, Liisa. / Quantitative determination of wood-derived soluble oligosaccharides by HPLC. In: Biotechnology Techniques. 1993 ; Vol. 7, No. 11. pp. 785 - 790.
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abstract = "Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.",
author = "Johanna Buchert and Matti Siika-aho and Michael Bailey and Jurgen Puls and Anne Valkeaj{\"a}rvi and Jaakko Pere and Liisa Viikari",
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Buchert, J, Siika-aho, M, Bailey, M, Puls, J, Valkeajärvi, A, Pere, J & Viikari, L 1993, 'Quantitative determination of wood-derived soluble oligosaccharides by HPLC', Biotechnology Techniques, vol. 7, no. 11, pp. 785 - 790. https://doi.org/10.1007/BF00153745

Quantitative determination of wood-derived soluble oligosaccharides by HPLC. / Buchert, Johanna; Siika-aho, Matti; Bailey, Michael; Puls, Jurgen; Valkeajärvi, Anne; Pere, Jaakko; Viikari, Liisa.

In: Biotechnology Techniques, Vol. 7, No. 11, 1993, p. 785 - 790.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Quantitative determination of wood-derived soluble oligosaccharides by HPLC

AU - Buchert, Johanna

AU - Siika-aho, Matti

AU - Bailey, Michael

AU - Puls, Jurgen

AU - Valkeajärvi, Anne

AU - Pere, Jaakko

AU - Viikari, Liisa

N1 - Project code: BIO2022

PY - 1993

Y1 - 1993

N2 - Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

AB - Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

U2 - 10.1007/BF00153745

DO - 10.1007/BF00153745

M3 - Article

VL - 7

SP - 785

EP - 790

JO - Biotechnology Techniques

JF - Biotechnology Techniques

SN - 0951-208X

IS - 11

ER -