Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.
Buchert, J., Siika-aho, M., Bailey, M., Puls, J., Valkeajärvi, A., Pere, J., & Viikari, L. (1993). Quantitative determination of wood-derived soluble oligosaccharides by HPLC. Biotechnology Techniques, 7(11), 785 - 790. https://doi.org/10.1007/BF00153745