Quantitative determination of wood-derived soluble oligosaccharides by HPLC

Johanna Buchert; Matti Siika-aho; Michael Bailey; Jurgen Puls; Anne Valkeajärvi; Jaakko Pere; Liisa Viikari

doi:10.1007/BF00153745

Quantitative determination of wood-derived soluble oligosaccharides by HPLC

Johanna Buchert, Matti Siika-aho, Michael Bailey, Jurgen Puls, Anne Valkeajärvi, Jaakko Pere, Liisa Viikari

Thünen Institute of Wood Research

Research output: Contribution to journal › Article › Scientific › peer-review

54 Citations (Scopus)

Abstract

Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

Original language	English
Pages (from-to)	785 - 790
Number of pages	6
Journal	Biotechnology Techniques
Volume	7
Issue number	11
DOIs	https://doi.org/10.1007/BF00153745
Publication status	Published - 1993
MoE publication type	A1 Journal article-refereed

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10.1007/BF00153745

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title = "Quantitative determination of wood-derived soluble oligosaccharides by HPLC",

abstract = "Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.",

author = "Johanna Buchert and Matti Siika-aho and Michael Bailey and Jurgen Puls and Anne Valkeaj{\"a}rvi and Jaakko Pere and Liisa Viikari",

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T1 - Quantitative determination of wood-derived soluble oligosaccharides by HPLC

AU - Buchert, Johanna

AU - Siika-aho, Matti

AU - Bailey, Michael

AU - Puls, Jurgen

AU - Valkeajärvi, Anne

AU - Pere, Jaakko

AU - Viikari, Liisa

N1 - Project code: BIO2022

PY - 1993

Y1 - 1993

N2 - Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

AB - Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

U2 - 10.1007/BF00153745

DO - 10.1007/BF00153745

M3 - Article

SN - 0951-208X

VL - 7

SP - 785

EP - 790

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 11

ER -

Quantitative determination of wood-derived soluble oligosaccharides by HPLC

Abstract

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