Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation

Robert Moulder, Tapio Lönnberg, Laura L. Elo, Jan-Jonas Filén, Eeva Rainio, Garry Corthals, Matej Orešič, Tuula A. Nyman, Tero Aittokallio, Riitta Lahesmaa (Corresponding Author)

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Abstract

We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4+ cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4+ cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4+ T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.
Original languageEnglish
Pages (from-to)1937-1953
JournalMolecular and Cellular Proteomics
Volume9
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Interleukin-4
Proteomics
Fetal Blood
Blood
Isotope Labeling
Th2 Cells
Proteins
Jurkat Cells
T-cells
Cell Nucleus
Isotopes
Labeling
Cell Differentiation
Cells
T-Lymphocytes
Peptides
Datasets

Cite this

Moulder, Robert ; Lönnberg, Tapio ; Elo, Laura L. ; Filén, Jan-Jonas ; Rainio, Eeva ; Corthals, Garry ; Orešič, Matej ; Nyman, Tuula A. ; Aittokallio, Tero ; Lahesmaa, Riitta. / Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation. In: Molecular and Cellular Proteomics. 2010 ; Vol. 9. pp. 1937-1953.
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title = "Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation",
abstract = "We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4+ cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated na{\"i}ve CD4+ cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4+ T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.",
author = "Robert Moulder and Tapio L{\"o}nnberg and Elo, {Laura L.} and Jan-Jonas Fil{\'e}n and Eeva Rainio and Garry Corthals and Matej Orešič and Nyman, {Tuula A.} and Tero Aittokallio and Riitta Lahesmaa",
year = "2010",
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language = "English",
volume = "9",
pages = "1937--1953",
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Moulder, R, Lönnberg, T, Elo, LL, Filén, J-J, Rainio, E, Corthals, G, Orešič, M, Nyman, TA, Aittokallio, T & Lahesmaa, R 2010, 'Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation', Molecular and Cellular Proteomics, vol. 9, pp. 1937-1953. https://doi.org/10.1074/mcp.M900483-MCP200

Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation. / Moulder, Robert; Lönnberg, Tapio; Elo, Laura L.; Filén, Jan-Jonas; Rainio, Eeva; Corthals, Garry; Orešič, Matej; Nyman, Tuula A.; Aittokallio, Tero; Lahesmaa, Riitta (Corresponding Author).

In: Molecular and Cellular Proteomics, Vol. 9, 2010, p. 1937-1953.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4 induced Th2 differentiation

AU - Moulder, Robert

AU - Lönnberg, Tapio

AU - Elo, Laura L.

AU - Filén, Jan-Jonas

AU - Rainio, Eeva

AU - Corthals, Garry

AU - Orešič, Matej

AU - Nyman, Tuula A.

AU - Aittokallio, Tero

AU - Lahesmaa, Riitta

PY - 2010

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N2 - We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4+ cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4+ cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4+ T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.

AB - We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4+ cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4+ cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4+ T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.

U2 - 10.1074/mcp.M900483-MCP200

DO - 10.1074/mcp.M900483-MCP200

M3 - Article

VL - 9

SP - 1937

EP - 1953

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

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