Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data

Maija-Liisa Mattinen, Kimmo Pääkkönen, Teemu Ikonen, Jeremy Craven, Torbjörn Drakenberg, Ritva Serimaa, Jonathan Waltho, Arto Annila

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

A new principle in constructing molecular complexes from the known high-resolution domain structures joining data from NMR and small-angle x-ray scattering (SAXS) measurements is described. Structure of calmodulin in complex with trifluoperazine was built from N- and C-terminal domains oriented based on residual dipolar couplings measured by NMR in a dilute liquid crystal, and the overall shape of the complex was derived from SAXS data. The residual dipolar coupling data serves to reduce angular degrees of freedom, and the small-angle scattering data serves to confine the translational degrees of freedom. The complex built by this method was found to be consistent with the known crystal structure. The study demonstrates how approximate tertiary structures of modular proteins or quaternary structures composed of subunits can be assembled from high-resolution structures of domains or subunits using mutually complementary NMR and SAXS data.
Original languageEnglish
Pages (from-to)1177-1183
JournalBiophysical Journal
Volume83
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

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X-Rays
Quaternary Protein Structure
Small Angle Scattering
Trifluoperazine
Liquid Crystals
Calmodulin
Tertiary Protein Structure

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Mattinen, M-L., Pääkkönen, K., Ikonen, T., Craven, J., Drakenberg, T., Serimaa, R., ... Annila, A. (2002). Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data. Biophysical Journal, 83(2), 1177-1183. https://doi.org/10.1016/S0006-3495(02)75241-7
Mattinen, Maija-Liisa ; Pääkkönen, Kimmo ; Ikonen, Teemu ; Craven, Jeremy ; Drakenberg, Torbjörn ; Serimaa, Ritva ; Waltho, Jonathan ; Annila, Arto. / Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data. In: Biophysical Journal. 2002 ; Vol. 83, No. 2. pp. 1177-1183.
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abstract = "A new principle in constructing molecular complexes from the known high-resolution domain structures joining data from NMR and small-angle x-ray scattering (SAXS) measurements is described. Structure of calmodulin in complex with trifluoperazine was built from N- and C-terminal domains oriented based on residual dipolar couplings measured by NMR in a dilute liquid crystal, and the overall shape of the complex was derived from SAXS data. The residual dipolar coupling data serves to reduce angular degrees of freedom, and the small-angle scattering data serves to confine the translational degrees of freedom. The complex built by this method was found to be consistent with the known crystal structure. The study demonstrates how approximate tertiary structures of modular proteins or quaternary structures composed of subunits can be assembled from high-resolution structures of domains or subunits using mutually complementary NMR and SAXS data.",
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Mattinen, M-L, Pääkkönen, K, Ikonen, T, Craven, J, Drakenberg, T, Serimaa, R, Waltho, J & Annila, A 2002, 'Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data', Biophysical Journal, vol. 83, no. 2, pp. 1177-1183. https://doi.org/10.1016/S0006-3495(02)75241-7

Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data. / Mattinen, Maija-Liisa; Pääkkönen, Kimmo; Ikonen, Teemu; Craven, Jeremy; Drakenberg, Torbjörn; Serimaa, Ritva; Waltho, Jonathan; Annila, Arto.

In: Biophysical Journal, Vol. 83, No. 2, 2002, p. 1177-1183.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Mattinen, Maija-Liisa

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AB - A new principle in constructing molecular complexes from the known high-resolution domain structures joining data from NMR and small-angle x-ray scattering (SAXS) measurements is described. Structure of calmodulin in complex with trifluoperazine was built from N- and C-terminal domains oriented based on residual dipolar couplings measured by NMR in a dilute liquid crystal, and the overall shape of the complex was derived from SAXS data. The residual dipolar coupling data serves to reduce angular degrees of freedom, and the small-angle scattering data serves to confine the translational degrees of freedom. The complex built by this method was found to be consistent with the known crystal structure. The study demonstrates how approximate tertiary structures of modular proteins or quaternary structures composed of subunits can be assembled from high-resolution structures of domains or subunits using mutually complementary NMR and SAXS data.

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Mattinen M-L, Pääkkönen K, Ikonen T, Craven J, Drakenberg T, Serimaa R et al. Quaternary structure built from subunits combining NMR and small-angle X-ray scattering data. Biophysical Journal. 2002;83(2):1177-1183. https://doi.org/10.1016/S0006-3495(02)75241-7