Rapid and Nondestructive Determination of Aleurone Content in Pearling Fractions of Barley by Near-Infrared (NIR) and Fluorescence Spectroscopies

Jans Petter Wold, Diego Airado-Rodríguez, Ann-Katrin Holtekjølen, Ulla Holopainen-Mantila, Stefan Sahlstrøm

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The aim of the present work was to develop and evaluate near-infrared (NIR) and fluorescence spectroscopies as rapid and potential online methods for determination of the amount of aleurone in pearling dust fractions of barley. Phytic acid was used as a marker for the aleurone layer. Different varieties of barley were pearled, and dust fractions were progressively taken out. Sample concentration of phytic acid varied in the range of 0.5-4.1 g/100 g, and highest concentrations were obtained in dust fractions for pearling degrees in the range of 15-25%. Regression models for phytic acid were developed with the same high correlations for NIR and fluorescence spectroscopies (R2 = 0.96) and prediction errors of ±0.16-0.18 g/100 g. The models performed well on a test set of pearling fractions from two other barley varieties. The techniques are rapid and nondestructive, which means that they can be used online in connection with industrial pearling systems.
Original languageEnglish
Pages (from-to)1813-1821
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Issue number8
Publication statusPublished - 1 Mar 2017
MoE publication typeA1 Journal article-refereed



  • aleurone
  • barley
  • fluorescence
  • NIR
  • pearling

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