Rapid detection of detergents and disinfectants in food factories

J. Lappalainen, S. Loikkanen, Marika Havana, Matti Karp, Anna-Maija Sjöberg, Gun Wirtanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Awareness of both microbial and chemical risks in the process industry has increased through the use of HACCP (Hazard Analysis Critical Control Point) systems for hygiene assessment. Reliable detection methods are needed to ensure the cleanliness of process lines and facilities. Chemical risks in food processing include e.g. tenside residues after insufficient rinsing of the process line. Knowledge of the chemical composition of cleaning agents and disinfectants is needed before performing chemical analyses. The ATP luminescence measurement is based on an enzymatic reaction and may be significantly affected by sanitising agents and disinfectants used for cleaning. The disinfectants can reduce the activity of the luciferase enzyme, but they can also act as ATP-releasing agents of contaminating microbes. The agents disrupt the cells but preserve ATP in measurable form, and therefore the correlation with traditional culture methods can be poor. If a rapid method is used to detect ATP, a control must be used and the effect of disinfectants eliminated to eliminate sources of error. A microbiological residue-testing method, nonspecific for residues, was developed. A total of 38 commercial cleaning agents and disinfectants of various types were measured using two microbiological methods, the Vibrio fischeri photobacteria test and the Micrococcus luteus inhibition zone technique. A small study in a food factory was also performed. The results show that microbial testing methods are extremely sensitive and that a need for this type of method exists.
Original languageEnglish
Title of host publication30th R3-Nordic Contamination Control Symposium
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages199-206
ISBN (Print)951-38-5268-7
Publication statusPublished - 1999
MoE publication typeA4 Article in a conference publication
Event30th R3-Nordic Contamination Control Symposium
- Helsinki, Finland
Duration: 30 May 19992 Jun 1999

Publication series

SeriesVTT Symposium
Number193
ISSN0357-9387

Conference

Conference30th R3-Nordic Contamination Control Symposium
CountryFinland
CityHelsinki
Period30/05/992/06/99

Fingerprint

Disinfectants
Detergents
Food
Adenosine Triphosphate
Aliivibrio fischeri
Photobacterium
Hazard Analysis and Critical Control Points
Micrococcus luteus
Luminescent Measurements
Food Handling
Luciferases
Hygiene
Surface-Active Agents
Industry
Research Design
Enzymes

Cite this

Lappalainen, J., Loikkanen, S., Havana, M., Karp, M., Sjöberg, A-M., & Wirtanen, G. (1999). Rapid detection of detergents and disinfectants in food factories. In 30th R3-Nordic Contamination Control Symposium (pp. 199-206). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 193
Lappalainen, J. ; Loikkanen, S. ; Havana, Marika ; Karp, Matti ; Sjöberg, Anna-Maija ; Wirtanen, Gun. / Rapid detection of detergents and disinfectants in food factories. 30th R3-Nordic Contamination Control Symposium. Espoo : VTT Technical Research Centre of Finland, 1999. pp. 199-206 (VTT Symposium; No. 193).
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abstract = "Awareness of both microbial and chemical risks in the process industry has increased through the use of HACCP (Hazard Analysis Critical Control Point) systems for hygiene assessment. Reliable detection methods are needed to ensure the cleanliness of process lines and facilities. Chemical risks in food processing include e.g. tenside residues after insufficient rinsing of the process line. Knowledge of the chemical composition of cleaning agents and disinfectants is needed before performing chemical analyses. The ATP luminescence measurement is based on an enzymatic reaction and may be significantly affected by sanitising agents and disinfectants used for cleaning. The disinfectants can reduce the activity of the luciferase enzyme, but they can also act as ATP-releasing agents of contaminating microbes. The agents disrupt the cells but preserve ATP in measurable form, and therefore the correlation with traditional culture methods can be poor. If a rapid method is used to detect ATP, a control must be used and the effect of disinfectants eliminated to eliminate sources of error. A microbiological residue-testing method, nonspecific for residues, was developed. A total of 38 commercial cleaning agents and disinfectants of various types were measured using two microbiological methods, the Vibrio fischeri photobacteria test and the Micrococcus luteus inhibition zone technique. A small study in a food factory was also performed. The results show that microbial testing methods are extremely sensitive and that a need for this type of method exists.",
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Lappalainen, J, Loikkanen, S, Havana, M, Karp, M, Sjöberg, A-M & Wirtanen, G 1999, Rapid detection of detergents and disinfectants in food factories. in 30th R3-Nordic Contamination Control Symposium. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 193, pp. 199-206, 30th R3-Nordic Contamination Control Symposium
, Helsinki, Finland, 30/05/99.

Rapid detection of detergents and disinfectants in food factories. / Lappalainen, J.; Loikkanen, S.; Havana, Marika; Karp, Matti; Sjöberg, Anna-Maija; Wirtanen, Gun.

30th R3-Nordic Contamination Control Symposium. Espoo : VTT Technical Research Centre of Finland, 1999. p. 199-206 (VTT Symposium; No. 193).

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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T1 - Rapid detection of detergents and disinfectants in food factories

AU - Lappalainen, J.

AU - Loikkanen, S.

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AU - Karp, Matti

AU - Sjöberg, Anna-Maija

AU - Wirtanen, Gun

PY - 1999

Y1 - 1999

N2 - Awareness of both microbial and chemical risks in the process industry has increased through the use of HACCP (Hazard Analysis Critical Control Point) systems for hygiene assessment. Reliable detection methods are needed to ensure the cleanliness of process lines and facilities. Chemical risks in food processing include e.g. tenside residues after insufficient rinsing of the process line. Knowledge of the chemical composition of cleaning agents and disinfectants is needed before performing chemical analyses. The ATP luminescence measurement is based on an enzymatic reaction and may be significantly affected by sanitising agents and disinfectants used for cleaning. The disinfectants can reduce the activity of the luciferase enzyme, but they can also act as ATP-releasing agents of contaminating microbes. The agents disrupt the cells but preserve ATP in measurable form, and therefore the correlation with traditional culture methods can be poor. If a rapid method is used to detect ATP, a control must be used and the effect of disinfectants eliminated to eliminate sources of error. A microbiological residue-testing method, nonspecific for residues, was developed. A total of 38 commercial cleaning agents and disinfectants of various types were measured using two microbiological methods, the Vibrio fischeri photobacteria test and the Micrococcus luteus inhibition zone technique. A small study in a food factory was also performed. The results show that microbial testing methods are extremely sensitive and that a need for this type of method exists.

AB - Awareness of both microbial and chemical risks in the process industry has increased through the use of HACCP (Hazard Analysis Critical Control Point) systems for hygiene assessment. Reliable detection methods are needed to ensure the cleanliness of process lines and facilities. Chemical risks in food processing include e.g. tenside residues after insufficient rinsing of the process line. Knowledge of the chemical composition of cleaning agents and disinfectants is needed before performing chemical analyses. The ATP luminescence measurement is based on an enzymatic reaction and may be significantly affected by sanitising agents and disinfectants used for cleaning. The disinfectants can reduce the activity of the luciferase enzyme, but they can also act as ATP-releasing agents of contaminating microbes. The agents disrupt the cells but preserve ATP in measurable form, and therefore the correlation with traditional culture methods can be poor. If a rapid method is used to detect ATP, a control must be used and the effect of disinfectants eliminated to eliminate sources of error. A microbiological residue-testing method, nonspecific for residues, was developed. A total of 38 commercial cleaning agents and disinfectants of various types were measured using two microbiological methods, the Vibrio fischeri photobacteria test and the Micrococcus luteus inhibition zone technique. A small study in a food factory was also performed. The results show that microbial testing methods are extremely sensitive and that a need for this type of method exists.

M3 - Conference article in proceedings

SN - 951-38-5268-7

T3 - VTT Symposium

SP - 199

EP - 206

BT - 30th R3-Nordic Contamination Control Symposium

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Lappalainen J, Loikkanen S, Havana M, Karp M, Sjöberg A-M, Wirtanen G. Rapid detection of detergents and disinfectants in food factories. In 30th R3-Nordic Contamination Control Symposium. Espoo: VTT Technical Research Centre of Finland. 1999. p. 199-206. (VTT Symposium; No. 193).