Reactivity of bacterial and fungal laccases with lignin under alkaline conditions

Raquel Moya, Päivi Saastamoinen, Manuel Hernández, Anna Suurnäkki, Enriqueta Arias, Maija-Liisa Mattinen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

The ability of Streptomyces ipomoea laccase to polymerize secoisolariciresinol lignan and technical lignins was assessed. The reactivity of S. ipomoea laccase was also compared to that of low redox fungal laccase from Melanocarpus albomyces using low molecular mass p-coumaric, ferulic and sinapic acid as well as natural (acetosyringone) and synthetic 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) mediators as substrates. Oxygen consumption measurement, MALDI-TOF MS and SEC were used to follow the enzymatic reactions at pH 7, 8, 9 and 10 at 30 °C and 50 °C. Polymerization of lignins and lignan by S. ipomoea laccase under alkaline reaction conditions was observed, and was enhanced in the presence of acetosyringone almost to the level obtained with M. albomyces laccase without mediator. Reactivities of the enzymes towards acetosyringone and TEMPO were similar, suggesting exploitation of the compounds and low redox laccase in lignin valorization under alkaline conditions. The results have scientific impact on basic research of laccases.
Original languageEnglish
Pages (from-to)10006-10012
JournalBioresource Technology
Volume102
Issue number21
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
lignin
Molecular mass
Lignans
ferulic acid
oxygen consumption
polymerization
Enzymes
Polymerization
enzyme
substrate
Oxygen
Acids
acid
Substrates
Oxidation-Reduction

Keywords

  • Laccase
  • Streptomyces ipomoea
  • Melanocarpus albomyces
  • lignin
  • alkali

Cite this

Moya, R., Saastamoinen, P., Hernández, M., Suurnäkki, A., Arias, E., & Mattinen, M-L. (2011). Reactivity of bacterial and fungal laccases with lignin under alkaline conditions. Bioresource Technology, 102(21), 10006-10012. https://doi.org/10.1016/j.biortech.2011.08.046
Moya, Raquel ; Saastamoinen, Päivi ; Hernández, Manuel ; Suurnäkki, Anna ; Arias, Enriqueta ; Mattinen, Maija-Liisa. / Reactivity of bacterial and fungal laccases with lignin under alkaline conditions. In: Bioresource Technology. 2011 ; Vol. 102, No. 21. pp. 10006-10012.
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Moya, R, Saastamoinen, P, Hernández, M, Suurnäkki, A, Arias, E & Mattinen, M-L 2011, 'Reactivity of bacterial and fungal laccases with lignin under alkaline conditions', Bioresource Technology, vol. 102, no. 21, pp. 10006-10012. https://doi.org/10.1016/j.biortech.2011.08.046

Reactivity of bacterial and fungal laccases with lignin under alkaline conditions. / Moya, Raquel; Saastamoinen, Päivi; Hernández, Manuel; Suurnäkki, Anna; Arias, Enriqueta; Mattinen, Maija-Liisa (Corresponding Author).

In: Bioresource Technology, Vol. 102, No. 21, 2011, p. 10006-10012.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The ability of Streptomyces ipomoea laccase to polymerize secoisolariciresinol lignan and technical lignins was assessed. The reactivity of S. ipomoea laccase was also compared to that of low redox fungal laccase from Melanocarpus albomyces using low molecular mass p-coumaric, ferulic and sinapic acid as well as natural (acetosyringone) and synthetic 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) mediators as substrates. Oxygen consumption measurement, MALDI-TOF MS and SEC were used to follow the enzymatic reactions at pH 7, 8, 9 and 10 at 30 °C and 50 °C. Polymerization of lignins and lignan by S. ipomoea laccase under alkaline reaction conditions was observed, and was enhanced in the presence of acetosyringone almost to the level obtained with M. albomyces laccase without mediator. Reactivities of the enzymes towards acetosyringone and TEMPO were similar, suggesting exploitation of the compounds and low redox laccase in lignin valorization under alkaline conditions. The results have scientific impact on basic research of laccases.

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Moya R, Saastamoinen P, Hernández M, Suurnäkki A, Arias E, Mattinen M-L. Reactivity of bacterial and fungal laccases with lignin under alkaline conditions. Bioresource Technology. 2011;102(21):10006-10012. https://doi.org/10.1016/j.biortech.2011.08.046