Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia

T. Yli-Mattila (Corresponding Author), S. Paavanen-Huhtala, M. Jestoi, P. Parikka, V. Hietaniemi, T. Gagkaeva, Tuija Sarlin, Auli Haikara, S. Laaksonen, A. Rizzo

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Abstract

TaqMan real-time quantitative PCR assays were developed for the accurate detection and quantification of DNA from Fusarium poae and F. graminearum species, which are able to produce trichothecenes. These and other PCR assays were used for the quantification of trichothecene-producing Fusarium fungi in cereal grains. A correlation was found between the levels of F. poae DNA and nivalenol and enniatins in barley and between the levels of F. graminearum DNA and deoxynivalenol in oats. The correlations between F. poae DNA and nivalenol and F. graminearum DNA and deoxynivalenol levels were higher than those between these mycotoxins and morphologically determined F. poae and F. graminearum/F. culmorum contamination levels. The use of F. poae specific primers and probe together with F. sporotrichioides/F. langsethiae specific primers and probe in a multiplex qPCR assay yielded results in accordance with those obtained using these primers and probes separately.
Original languageEnglish
Pages (from-to)243-260
Number of pages18
JournalArchives of Phytopathology and Plant Protection
Volume41
Issue number4
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

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Keywords

  • Barley
  • deoxynivalenol
  • enniatins
  • nivalenol
  • oats
  • TaqMan PCR

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