Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia

T. Yli-Mattila (Corresponding Author), S. Paavanen-Huhtala, M. Jestoi, P. Parikka, V. Hietaniemi, T. Gagkaeva, Tuija Sarlin, Auli Haikara, S. Laaksonen, A. Rizzo

Research output: Contribution to journalArticleScientificpeer-review

81 Citations (Scopus)

Abstract

TaqMan real-time quantitative PCR assays were developed for the accurate detection and quantification of DNA from Fusarium poae and F. graminearum species, which are able to produce trichothecenes. These and other PCR assays were used for the quantification of trichothecene-producing Fusarium fungi in cereal grains. A correlation was found between the levels of F. poae DNA and nivalenol and enniatins in barley and between the levels of F. graminearum DNA and deoxynivalenol in oats. The correlations between F. poae DNA and nivalenol and F. graminearum DNA and deoxynivalenol levels were higher than those between these mycotoxins and morphologically determined F. poae and F. graminearum/F. culmorum contamination levels. The use of F. poae specific primers and probe together with F. sporotrichioides/F. langsethiae specific primers and probe in a multiplex qPCR assay yielded results in accordance with those obtained using these primers and probes separately.
Original languageEnglish
Pages (from-to)243-260
Number of pages18
JournalArchives of Phytopathology and Plant Protection
Volume41
Issue number4
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Fusarium sporotrichioides
Fusarium poae
Fusarium graminearum
Russia
Finland
small cereal grains
quantitative polymerase chain reaction
DNA
nivalenol
trichothecenes
deoxynivalenol
assays
mycotoxins
Fusarium
oats
barley
fungi

Keywords

  • Barley
  • deoxynivalenol
  • enniatins
  • nivalenol
  • oats
  • TaqMan PCR

Cite this

Yli-Mattila, T. ; Paavanen-Huhtala, S. ; Jestoi, M. ; Parikka, P. ; Hietaniemi, V. ; Gagkaeva, T. ; Sarlin, Tuija ; Haikara, Auli ; Laaksonen, S. ; Rizzo, A. / Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. In: Archives of Phytopathology and Plant Protection. 2008 ; Vol. 41, No. 4. pp. 243-260.
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abstract = "TaqMan real-time quantitative PCR assays were developed for the accurate detection and quantification of DNA from Fusarium poae and F. graminearum species, which are able to produce trichothecenes. These and other PCR assays were used for the quantification of trichothecene-producing Fusarium fungi in cereal grains. A correlation was found between the levels of F. poae DNA and nivalenol and enniatins in barley and between the levels of F. graminearum DNA and deoxynivalenol in oats. The correlations between F. poae DNA and nivalenol and F. graminearum DNA and deoxynivalenol levels were higher than those between these mycotoxins and morphologically determined F. poae and F. graminearum/F. culmorum contamination levels. The use of F. poae specific primers and probe together with F. sporotrichioides/F. langsethiae specific primers and probe in a multiplex qPCR assay yielded results in accordance with those obtained using these primers and probes separately.",
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Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. / Yli-Mattila, T. (Corresponding Author); Paavanen-Huhtala, S.; Jestoi, M.; Parikka, P.; Hietaniemi, V.; Gagkaeva, T.; Sarlin, Tuija; Haikara, Auli; Laaksonen, S.; Rizzo, A.

In: Archives of Phytopathology and Plant Protection, Vol. 41, No. 4, 2008, p. 243-260.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Jestoi, M.

AU - Parikka, P.

AU - Hietaniemi, V.

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AU - Rizzo, A.

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AB - TaqMan real-time quantitative PCR assays were developed for the accurate detection and quantification of DNA from Fusarium poae and F. graminearum species, which are able to produce trichothecenes. These and other PCR assays were used for the quantification of trichothecene-producing Fusarium fungi in cereal grains. A correlation was found between the levels of F. poae DNA and nivalenol and enniatins in barley and between the levels of F. graminearum DNA and deoxynivalenol in oats. The correlations between F. poae DNA and nivalenol and F. graminearum DNA and deoxynivalenol levels were higher than those between these mycotoxins and morphologically determined F. poae and F. graminearum/F. culmorum contamination levels. The use of F. poae specific primers and probe together with F. sporotrichioides/F. langsethiae specific primers and probe in a multiplex qPCR assay yielded results in accordance with those obtained using these primers and probes separately.

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