Recent advances in homogenous immunoassays based on resonance energy transfer

Kristiina Takkinen, Aurelija Žvirblienė (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Development of homogeneous immunoassays based on Förster resonance energy transfer (FRET), chemiluminescence resonance energy transfer (CRET) or bioluminescence resonance energy transfer (BRET) enables a one-step, rapid and direct detection of analytes as compared to the multistep, time-consuming heterogeneous immunoassays. Antibody fragments such as Fab or F(ab)2 are extensively exploited in both competitive and non-competitive formats to circumvent the size limitations characteristic of full-length antibodies. Semiconductor fluorescent nanocrystals, quantum dots are becoming increasingly popular as energy acceptors due to their beneficial optical properties as compared to organic dyes. These and other technical advances open new ways for using homogenous immunoassays in a variety of bioanalytical applications including detection of protein biomarkers, hormones, drugs of abuse, food and environmental toxins.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume55
DOIs
Publication statusPublished - Feb 2019
MoE publication typeNot Eligible

Fingerprint

Energy Transfer
Immunoassay
Energy transfer
Quantum Dots
Antibodies
Bioluminescence
Immunoglobulin Fragments
Chemiluminescence
Hormones
Street Drugs
Biomarkers
Luminescence
Nanocrystals
Semiconductor quantum dots
Coloring Agents
Dyes
Optical properties
Semiconductor materials
Proteins
Food

Cite this

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Recent advances in homogenous immunoassays based on resonance energy transfer. / Takkinen, Kristiina; Žvirblienė, Aurelija (Corresponding Author).

In: Current Opinion in Biotechnology, Vol. 55, 02.2019, p. 16-22.

Research output: Contribution to journalArticleScientificpeer-review

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