Recent advances in homogenous immunoassays based on resonance energy transfer

Kristiina Takkinen, Aurelija Žvirblienė (Corresponding Author)

    Research output: Contribution to journalReview ArticleResearchpeer-review

    Abstract

    Development of homogeneous immunoassays based on Förster resonance energy transfer (FRET), chemiluminescence resonance energy transfer (CRET) or bioluminescence resonance energy transfer (BRET) enables a one-step, rapid and direct detection of analytes as compared to the multistep, time-consuming heterogeneous immunoassays. Antibody fragments such as Fab or F(ab)2 are extensively exploited in both competitive and non-competitive formats to circumvent the size limitations characteristic of full-length antibodies. Semiconductor fluorescent nanocrystals, quantum dots are becoming increasingly popular as energy acceptors due to their beneficial optical properties as compared to organic dyes. These and other technical advances open new ways for using homogenous immunoassays in a variety of bioanalytical applications including detection of protein biomarkers, hormones, drugs of abuse, food and environmental toxins.

    LanguageEnglish
    Pages16-22
    Number of pages7
    JournalCurrent Opinion in Biotechnology
    Volume55
    DOIs
    Publication statusPublished - Feb 2019
    MoE publication typeNot Eligible

    Fingerprint

    Energy Transfer
    Immunoassay
    Energy transfer
    Quantum Dots
    Antibodies
    Bioluminescence
    Immunoglobulin Fragments
    Chemiluminescence
    Hormones
    Street Drugs
    Biomarkers
    Luminescence
    Nanocrystals
    Semiconductor quantum dots
    Coloring Agents
    Dyes
    Optical properties
    Semiconductor materials
    Proteins
    Food

    OKM Publication Types

    • A1 Refereed journal article

    OKM Open Access Status

    • 0 Not Open Access

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering

    Cite this

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    Recent advances in homogenous immunoassays based on resonance energy transfer. / Takkinen, Kristiina; Žvirblienė, Aurelija (Corresponding Author).

    In: Current Opinion in Biotechnology, Vol. 55, 02.2019, p. 16-22.

    Research output: Contribution to journalReview ArticleResearchpeer-review

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