Recent advances in homogenous immunoassays based on resonance energy transfer

Kristiina Takkinen; Aurelija Žvirblienė

doi:10.1016/j.copbio.2018.07.003

Recent advances in homogenous immunoassays based on resonance energy transfer

Kristiina Takkinen, Aurelija Žvirblienė (Corresponding Author)

Vilnius University

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

Development of homogeneous immunoassays based on Förster resonance energy transfer (FRET), chemiluminescence resonance energy transfer (CRET) or bioluminescence resonance energy transfer (BRET) enables a one-step, rapid and direct detection of analytes as compared to the multistep, time-consuming heterogeneous immunoassays. Antibody fragments such as Fab or F(ab)₂ are extensively exploited in both competitive and non-competitive formats to circumvent the size limitations characteristic of full-length antibodies. Semiconductor fluorescent nanocrystals, quantum dots are becoming increasingly popular as energy acceptors due to their beneficial optical properties as compared to organic dyes. These and other technical advances open new ways for using homogenous immunoassays in a variety of bioanalytical applications including detection of protein biomarkers, hormones, drugs of abuse, food and environmental toxins.

Original language	English
Pages (from-to)	16-22
Journal	Current Opinion in Biotechnology
Volume	55
DOIs	https://doi.org/10.1016/j.copbio.2018.07.003
Publication status	Published - Feb 2019
MoE publication type	A1 Journal article-refereed

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10.1016/j.copbio.2018.07.003

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Recent advances in homogenous immunoassays based on resonance energy transfer

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