Recombinant Amb a 1 expression

Georg Schmidt, N. Wopfner, F. Ferreira, Anneli Ritala

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    The number of identified pollen allergens, especially homologues belonging to the group of pectate lyases, is constantly rising. The main allergenic protein of common ragweed (Ambrosia artemisiifolia) Amb a 1 resembles the most prominent specimen. The major allergens from pollen of cedar, juniper and cypress species Jun a 1 (Juniperus ashei), Cry j 1 (Cryptomeria japonica), Cup a 1 (Cupressus arizonica) and Cha o 1 (Chamaecyparis obtuse) as well as Art v 6 from Mugwort (Artemisia vulgaris) also belong to the family of pectate lyases. Pectate lyases themselves resemble a large group of enzymes not only expressed in ripening fruits and in plant pathogenic bacteria, but also in pollen. These enzymes are able to digest the pectin or pectate envelope of plant cells. Although enzymatic activity is not shown for all above mentioned allergens, the high homology on aminoacid level and the cross-reactivity of patient s sera especially within the conifers unify this group. In the U.S. about 50% of all allergic individuals (including patients with food, dust, mite and other allergies) suffer from ragweed pollinosis (hay fever). This shows the magnitude of the ragweed problem. Since ragweed already started invading parts of France, Hungary, Germany, Finland and Austria, this very potent, allergy provoking plant will soon cause big problems in Europe, too. Accurate standardization of natural pollen extracts is a difficult task. Thus there is a need for reliable and effective diagnostic and therapeutic tools for allergy treatment. Therefore the production of soluble recombinant allergens of the pectate lyase group is an important task. For this purpose we were evaluating the plant-based expression systems for Amb a1.
    Original languageEnglish
    Title of host publicationPlants for Human Health in the Post-Genome Era
    Subtitle of host publicationPSE Congress
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    Pages125-125
    ISBN (Electronic)978-951-38-6322-7
    ISBN (Print)978-951-38-6321-0
    Publication statusPublished - 2007
    EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
    Duration: 26 Aug 200729 Aug 2007

    Publication series

    SeriesVTT Symposium
    Number249

    Conference

    ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
    Country/TerritoryFinland
    CityHelsinki
    Period26/08/0729/08/07

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