Recombinant brewer's yeast strains suitable for accelerated brewing

M. L. Suihko, K. Blomqvist, M. Penttilä, R. Gisler, J. Knowles

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)


Four brewer's yeast strains carrying the α-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The α-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.

Original languageEnglish
Pages (from-to)285-300
Number of pages16
JournalJournal of Biotechnology
Issue number3-4
Publication statusPublished - 1 Jan 1990
MoE publication typeA1 Journal article-refereed


  • Accelerated brewing
  • Brewer's yeast
  • Recombinant strains
  • α-Acetolactate decarboxylase

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