Recombinant brewer's yeast strains suitable for accelerated brewing

Maija-Liisa Suihko; Kristina Blomqvist; Merja Penttilä; Raija Gisler; Jonathan Knowles

doi:10.1016/0168-1656(90)90113-P

Recombinant brewer's yeast strains suitable for accelerated brewing

Maija-Liisa Suihko^*
, Kristina Blomqvist
, Merja Penttilä
, Raija Gisler
, Jonathan Knowles

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

30 Citations (Scopus)

Abstract

Four brewer's yeast strains carrying the α-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The α-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.

Original language	English
Pages (from-to)	285-300
Journal	Journal of Biotechnology
Volume	14
Issue number	3-4
DOIs	https://doi.org/10.1016/0168-1656(90)90113-P
Publication status	Published - 1 Jan 1990
MoE publication type	A1 Journal article-refereed

Funding

This research was financed by the Technology Development Centre (TEKES), Oy Panimolaboratorio-Bryggerilaboratorium Ab and Stiftelsen Svensk Etanolutveck-ling. We wish to thank warmly all those involved in this work at VTT Biotechnical Laboratory.

Keywords

Accelerated brewing
Brewer's yeast
Recombinant strains
α-Acetolactate decarboxylase

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10.1016/0168-1656(90)90113-P

Cite this

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abstract = "Four brewer's yeast strains carrying the α-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The α-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.",

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AU - Suihko, Maija-Liisa

AU - Blomqvist, Kristina

AU - Penttilä, Merja

AU - Gisler, Raija

AU - Knowles, Jonathan

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Four brewer's yeast strains carrying the α-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The α-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.

AB - Four brewer's yeast strains carrying the α-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The α-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.

KW - Accelerated brewing

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KW - Recombinant strains

KW - α-Acetolactate decarboxylase

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ER -

Recombinant brewer's yeast strains suitable for accelerated brewing

Abstract

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Keywords

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