Recombinant process for producing steryl esterase

Hanna Kontkanen

Research output: Contribution to journalArticleProfessional

Abstract

Researchers at the Technical Research Center (VTT) of Finland have isolated the ste1 gene encoding a steryl esterase from Melanocarpus albomyces fungus and used it to transform other organisms to produce the enzyme. First, the group used the gene, minus an intron that the wild type gene contains, to transform Pichia pastoris yeast under an inducible aldehyde oxidase (AOX1) promoter. Because production level was low, the group then expressed the gene in the wood rot fungus (Trichoderma reesei) under the inducible cellobiohydrolase (cbh1) promoter. Although production was satisfactory, about 60% of the enzyme was bound to the mycelium of the fungus or to solid particles in the medium or was in the form of aggregates. However, treatment with Trion X-100 recovered active enzyme.
Original languageEnglish
Article number8
JournalIndustrial Bioprocessing
Volume28
Issue number5
Publication statusPublished - 2006
MoE publication typeD1 Article in a trade journal

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Esterases
Fungi
Enzymes
Genes
Aldehyde Oxidase
Cellulose 1,4-beta-Cellobiosidase
Gene encoding
Aldehydes
Yeast
Introns
Trichoderma
Pichia
Mycelium
Wood
Finland
Yeasts
Research Personnel
Research

Cite this

Kontkanen, Hanna. / Recombinant process for producing steryl esterase. In: Industrial Bioprocessing. 2006 ; Vol. 28, No. 5.
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abstract = "Researchers at the Technical Research Center (VTT) of Finland have isolated the ste1 gene encoding a steryl esterase from Melanocarpus albomyces fungus and used it to transform other organisms to produce the enzyme. First, the group used the gene, minus an intron that the wild type gene contains, to transform Pichia pastoris yeast under an inducible aldehyde oxidase (AOX1) promoter. Because production level was low, the group then expressed the gene in the wood rot fungus (Trichoderma reesei) under the inducible cellobiohydrolase (cbh1) promoter. Although production was satisfactory, about 60{\%} of the enzyme was bound to the mycelium of the fungus or to solid particles in the medium or was in the form of aggregates. However, treatment with Trion X-100 recovered active enzyme.",
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Recombinant process for producing steryl esterase. / Kontkanen, Hanna.

In: Industrial Bioprocessing, Vol. 28, No. 5, 8, 2006.

Research output: Contribution to journalArticleProfessional

TY - JOUR

T1 - Recombinant process for producing steryl esterase

AU - Kontkanen, Hanna

PY - 2006

Y1 - 2006

N2 - Researchers at the Technical Research Center (VTT) of Finland have isolated the ste1 gene encoding a steryl esterase from Melanocarpus albomyces fungus and used it to transform other organisms to produce the enzyme. First, the group used the gene, minus an intron that the wild type gene contains, to transform Pichia pastoris yeast under an inducible aldehyde oxidase (AOX1) promoter. Because production level was low, the group then expressed the gene in the wood rot fungus (Trichoderma reesei) under the inducible cellobiohydrolase (cbh1) promoter. Although production was satisfactory, about 60% of the enzyme was bound to the mycelium of the fungus or to solid particles in the medium or was in the form of aggregates. However, treatment with Trion X-100 recovered active enzyme.

AB - Researchers at the Technical Research Center (VTT) of Finland have isolated the ste1 gene encoding a steryl esterase from Melanocarpus albomyces fungus and used it to transform other organisms to produce the enzyme. First, the group used the gene, minus an intron that the wild type gene contains, to transform Pichia pastoris yeast under an inducible aldehyde oxidase (AOX1) promoter. Because production level was low, the group then expressed the gene in the wood rot fungus (Trichoderma reesei) under the inducible cellobiohydrolase (cbh1) promoter. Although production was satisfactory, about 60% of the enzyme was bound to the mycelium of the fungus or to solid particles in the medium or was in the form of aggregates. However, treatment with Trion X-100 recovered active enzyme.

M3 - Article

VL - 28

JO - Industrial Bioprocessing

JF - Industrial Bioprocessing

SN - 1056-7194

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