Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 434-444 |
| Journal | Plant Biotechnology Journal |
| Volume | 9 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- molecular farming
- recombinant protein production
- transgenic plants
- transient expression
- erythropoietin
- interleukin-10
- alfa-amylase
- Pseudomonas aeruginosa
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Recombinant protein production in a variety of Nicotiana hosts : A comparative analysis. / Conley, Andrew; Zhu, Hong; Le, Linda C.; Jevnikar, Anthony M.; Lee, Byong H.; Brandle, Jim E.; Menassa, Rima (Corresponding Author).
In: Plant Biotechnology Journal, Vol. 9, No. 4, 2011, p. 434-444.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Recombinant protein production in a variety of Nicotiana hosts
T2 - A comparative analysis
AU - Conley, Andrew
AU - Zhu, Hong
AU - Le, Linda C.
AU - Jevnikar, Anthony M.
AU - Lee, Byong H.
AU - Brandle, Jim E.
AU - Menassa, Rima
PY - 2011
Y1 - 2011
N2 - Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin‐10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable α‐amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.
AB - Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin‐10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable α‐amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.
KW - molecular farming
KW - recombinant protein production
KW - transgenic plants
KW - transient expression
KW - erythropoietin
KW - interleukin-10
KW - alfa-amylase
KW - Pseudomonas aeruginosa
U2 - 10.1111/j.1467-7652.2010.00563.x
DO - 10.1111/j.1467-7652.2010.00563.x
M3 - Article
VL - 9
SP - 434
EP - 444
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
SN - 1467-7644
IS - 4
ER -