Reconstruction of stratum corneum profile of porcine ear skin after tape stripping using UV/VIS spectroscopy

Alexey P. Popov, Jürgen Lademann, Alexander V. Priezzhev, Risto Myllylä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Stratum corneum (horny layer) is a superficial skin layer consisting of dead cells. To reveal in-depth penetration profiles of substances topically applied onto skin surface, a minimally invasive method called tape stripping is widely used. It introduces consecutive removal of micrometer-thick cell layers of stratum corneum from the same treated skin area using an adhesive tape. Prerequisite to the substance penetration profile is the reconstruction of the removed stratum corneum by analyzing the amount of corneocytes (cells of stratum corneum from) stuck to each tape strip. Before application in vivo on humans, porcine skin is often used for such kind of studies. In this paper, we present results of the experiments with porcine skin in vitro (ears of freshly slaughtered pigs) and compare them with those carried out on humans in vivo (flexor forearm) taken from references. As we proved experimentally, there is a linear dependence between the absorbance (equals to logarithm of inverse transmittance) and thickness of the corneocytes on tape strips for all wavelength of the investigated region (300-1050 nm). Dependence of the cumulative absorbance of removed stratum corneum on tape strip number can be satisfactory fitted by an exponential function. This relationship allows evaluation of the relative share of the removed stratum corneum without complete removal of the layer. All the obtained results correlate well with those obtained on humans.

Original languageEnglish
Title of host publicationDiagnostic Optical Spectroscopy in Biomedicine IV
EditorsDietrich Schweitzer, Maryann Fitzmaurice
PublisherOptical Society of America OSA
ChapterPOSTER SESSION (PS)
ISBN (Print)978-0-81946-772-0
DOIs
Publication statusPublished - 1 Jan 2007
MoE publication typeNot Eligible
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: 17 Jun 200717 Jun 2007

Publication series

SeriesProgress in Biomedical Optics and Imaging
Number41
Volume8
ISSN1605-7422
SeriesProceedings of SPIE
Volume6628
ISSN0277-786X

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2007
CountryGermany
CityMunich
Period17/06/0717/06/07

Keywords

  • Porcine skin
  • Spectroscopy
  • Stratum corenum
  • Tape stripping

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    Popov, A. P., Lademann, J., Priezzhev, A. V., & Myllylä, R. (2007). Reconstruction of stratum corneum profile of porcine ear skin after tape stripping using UV/VIS spectroscopy. In D. Schweitzer, & M. Fitzmaurice (Eds.), Diagnostic Optical Spectroscopy in Biomedicine IV [6628_70] Optical Society of America OSA. Progress in Biomedical Optics and Imaging, No. 41, Vol.. 8, Proceedings of SPIE, Vol.. 6628 https://doi.org/10.1364/ECBO.2007.6628_70, https://doi.org/10.1117/12.729525