Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts

John Jairo Aguilera-Correa; Liisa Nohynek; Hanna Leena Alakomi; Jaime Esteban; Kirsi Marja Oksman-Caldentey; Riitta Puupponen-Pimiä; Teemu J. Kinnari; Ramon Perez-Tanoira

doi:10.3389/fcimb.2023.1176755

Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts

John Jairo Aguilera-Correa
, Liisa Nohynek
, Hanna Leena Alakomi
, Jaime Esteban
, Kirsi Marja Oksman-Caldentey
, Riitta Puupponen-Pimiä
, Teemu J. Kinnari^*
, Ramon Perez-Tanoira^*

^*Corresponding author for this work

BA53 Industrial biotechnology and food

Research output: Contribution to journal › Article › Scientific › peer-review

3 Citations (Scopus)

Abstract

Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by Staphylococcus aureus (S. aureus). More specifically, methicillin-resistant S. aureus (MRSA) infection poses a serious threat to global health. Therefore, developing new antibacterial agents to address drug resistance are urgently needed. Compounds derived from natural berries have shown a strong antimicrobial potential. Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (Rubus chamaemorus) and raspberry (Rubus idaeus), on the development of an MRSA biofilm and as treatment on a mature MRSA biofilm. Furthermore, we evaluated the ability of two cloudberry seed-coat fractions, hydrothermal extract and ethanol extract, and the wet-milled hydrothermal extract of a raspberry press cake to inhibit and treat biofilm development in a wound-like medium. To do so, we used a model strain and two clinical strains isolated from infected patients. Results: All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. Discussion: The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.

Original language	English
Article number	1176755
Number of pages	8
Journal	Frontiers in cellular and infection microbiology
Volume	13
DOIs	https://doi.org/10.3389/fcimb.2023.1176755
Publication status	Published - 23 Jun 2023
MoE publication type	A1 Journal article-refereed

Funding

The research presented in the manuscript has got public funding from Business Finland and from internal funding of VTT and Helsinki University Hospital. J.E. received travel grants from Pfizer and conference fees from Biomérieux and Heraeus. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antimicrobial
berry extract
biofilm
methicillin-resistant Staphylococcus aureus (MRSA)
surgical infection

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10.3389/fcimb.2023.1176755Licence: CC BY

Cite this

Aguilera-Correa, J. J., Nohynek, L., Alakomi, H. L., Esteban, J., Oksman-Caldentey, K. M., Puupponen-Pimiä, R., Kinnari, T. J., & Perez-Tanoira, R. (2023). Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts. Frontiers in cellular and infection microbiology, 13, Article 1176755. https://doi.org/10.3389/fcimb.2023.1176755

@article{7ebbbfdc5ca04c719f9f5e99998b836c,

title = "Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts",

abstract = "Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by Staphylococcus aureus (S. aureus). More specifically, methicillin-resistant S. aureus (MRSA) infection poses a serious threat to global health. Therefore, developing new antibacterial agents to address drug resistance are urgently needed. Compounds derived from natural berries have shown a strong antimicrobial potential. Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (Rubus chamaemorus) and raspberry (Rubus idaeus), on the development of an MRSA biofilm and as treatment on a mature MRSA biofilm. Furthermore, we evaluated the ability of two cloudberry seed-coat fractions, hydrothermal extract and ethanol extract, and the wet-milled hydrothermal extract of a raspberry press cake to inhibit and treat biofilm development in a wound-like medium. To do so, we used a model strain and two clinical strains isolated from infected patients. Results: All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. Discussion: The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.",

keywords = "antimicrobial, berry extract, biofilm, methicillin-resistant Staphylococcus aureus (MRSA), surgical infection",

author = "Aguilera-Correa, \{John Jairo\} and Liisa Nohynek and Alakomi, \{Hanna Leena\} and Jaime Esteban and Oksman-Caldentey, \{Kirsi Marja\} and Riitta Puupponen-Pimi{\"a} and Kinnari, \{Teemu J.\} and Ramon Perez-Tanoira",

note = "Funding Information: The research presented in the manuscript has got public funding from Business Finland and from internal funding of VTT and Helsinki University Hospital. J.E. received travel grants from Pfizer and conference fees from Biom{\'e}rieux and Heraeus. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Publisher Copyright: Copyright {\textcopyright} 2023 Aguilera-Correa, Nohynek, Alakomi, Esteban, Oksman-Caldentey, Puupponen-Pimi{\"a}, Kinnari and Perez-Tanoira.",

year = "2023",

month = jun,

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doi = "10.3389/fcimb.2023.1176755",

language = "English",

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journal = "Frontiers in cellular and infection microbiology",

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TY - JOUR

T1 - Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts

AU - Aguilera-Correa, John Jairo

AU - Nohynek, Liisa

AU - Alakomi, Hanna Leena

AU - Esteban, Jaime

AU - Oksman-Caldentey, Kirsi Marja

AU - Puupponen-Pimiä, Riitta

AU - Kinnari, Teemu J.

AU - Perez-Tanoira, Ramon

N1 - Funding Information: The research presented in the manuscript has got public funding from Business Finland and from internal funding of VTT and Helsinki University Hospital. J.E. received travel grants from Pfizer and conference fees from Biomérieux and Heraeus. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Publisher Copyright: Copyright © 2023 Aguilera-Correa, Nohynek, Alakomi, Esteban, Oksman-Caldentey, Puupponen-Pimiä, Kinnari and Perez-Tanoira.

PY - 2023/6/23

Y1 - 2023/6/23

N2 - Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by Staphylococcus aureus (S. aureus). More specifically, methicillin-resistant S. aureus (MRSA) infection poses a serious threat to global health. Therefore, developing new antibacterial agents to address drug resistance are urgently needed. Compounds derived from natural berries have shown a strong antimicrobial potential. Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (Rubus chamaemorus) and raspberry (Rubus idaeus), on the development of an MRSA biofilm and as treatment on a mature MRSA biofilm. Furthermore, we evaluated the ability of two cloudberry seed-coat fractions, hydrothermal extract and ethanol extract, and the wet-milled hydrothermal extract of a raspberry press cake to inhibit and treat biofilm development in a wound-like medium. To do so, we used a model strain and two clinical strains isolated from infected patients. Results: All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. Discussion: The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.

AB - Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by Staphylococcus aureus (S. aureus). More specifically, methicillin-resistant S. aureus (MRSA) infection poses a serious threat to global health. Therefore, developing new antibacterial agents to address drug resistance are urgently needed. Compounds derived from natural berries have shown a strong antimicrobial potential. Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (Rubus chamaemorus) and raspberry (Rubus idaeus), on the development of an MRSA biofilm and as treatment on a mature MRSA biofilm. Furthermore, we evaluated the ability of two cloudberry seed-coat fractions, hydrothermal extract and ethanol extract, and the wet-milled hydrothermal extract of a raspberry press cake to inhibit and treat biofilm development in a wound-like medium. To do so, we used a model strain and two clinical strains isolated from infected patients. Results: All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. Discussion: The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.

KW - antimicrobial

KW - berry extract

KW - biofilm

KW - methicillin-resistant Staphylococcus aureus (MRSA)

KW - surgical infection

UR - https://www.scopus.com/pages/publications/85164109460

U2 - 10.3389/fcimb.2023.1176755

DO - 10.3389/fcimb.2023.1176755

M3 - Article

C2 - 37424779

AN - SCOPUS:85164109460

SN - 2235-2988

VL - 13

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

M1 - 1176755

ER -

Reduction of methicillin-resistant Staphylococcus aureus biofilm growth and development using arctic berry extracts

Abstract

Funding

UN SDGs

Keywords

Access to Document

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