TY - JOUR
T1 - Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study
AU - Mykkänen, O.T.
AU - Kirjavainen, P.V.
AU - Pulkkinen, L.
AU - Mykkänen, H.
AU - Törrönen, R.
AU - Kolehmainen, M.
AU - Adriaens, M.
AU - Laaksonen, D.E.
AU - Puupponen-Pimiä, Riitta
AU - Poutanen, Kaisa
PY - 2014
Y1 - 2014
N2 - Obesity and its consequences on cardiometabolic health
have been associated to low-grade inflammation.
Anthocyanins are anti-inflammatory compounds in diet, and
the major group of polyphenols found in wild European
blueberries, bilberries (Vaccinium myrtillus). We have
studied the effects of a bilberry-rich diet on
inflammation and gene expression profile in peripheral
blood mononuclear cells (PBMCs) in subjects with features
of the metabolic syndrome. In a randomized, controlled
dietary intervention study the bilberry group (n=15)
consumed bilberries or berry products equivalent to 400 g
of fresh berries daily for 8 weeks, while the
participants in the control group (n=12) maintained their
habitual diets. Inflammatory markers were measured before
and after the intervention. The microarray profiling was
done from 3 subjects in the bilberry group and further
QPCR expression analyses from all subjects in both groups
at both timepoints. From 50 differentially expressed
transcripts for mRNA (P>0.005), five candidate genes;
WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly
selected for QPCR analyses. To further explore the
interplay of dietary change and the affected pathways
additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2),
TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell
receptor pathway) MMD, CCR2 (Monocyte and macrophage
associated genes) were selected for QPCR analyses.
Bilberries tended to decrease plasma concentrations of
hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An
inflammation score based on these markers and TNF-Alpha
showed different trends between the bilberry and control
groups. These changes were accompanied by differential
expression in PBMCs of MMD and CCR2, transcripts
associated with monocyte activation and recruitment,
respectively. Our results indicate that high regular
bilberry intake may reduce endotoxemia and chronic
inflammation, the latter especially by directing the
immunity away from overactive innate cell mediated
responsiveness. Bilberry consumption may therefore
decrease the long term risk of cardiovascular and
metabolic diseases
AB - Obesity and its consequences on cardiometabolic health
have been associated to low-grade inflammation.
Anthocyanins are anti-inflammatory compounds in diet, and
the major group of polyphenols found in wild European
blueberries, bilberries (Vaccinium myrtillus). We have
studied the effects of a bilberry-rich diet on
inflammation and gene expression profile in peripheral
blood mononuclear cells (PBMCs) in subjects with features
of the metabolic syndrome. In a randomized, controlled
dietary intervention study the bilberry group (n=15)
consumed bilberries or berry products equivalent to 400 g
of fresh berries daily for 8 weeks, while the
participants in the control group (n=12) maintained their
habitual diets. Inflammatory markers were measured before
and after the intervention. The microarray profiling was
done from 3 subjects in the bilberry group and further
QPCR expression analyses from all subjects in both groups
at both timepoints. From 50 differentially expressed
transcripts for mRNA (P>0.005), five candidate genes;
WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly
selected for QPCR analyses. To further explore the
interplay of dietary change and the affected pathways
additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2),
TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell
receptor pathway) MMD, CCR2 (Monocyte and macrophage
associated genes) were selected for QPCR analyses.
Bilberries tended to decrease plasma concentrations of
hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An
inflammation score based on these markers and TNF-Alpha
showed different trends between the bilberry and control
groups. These changes were accompanied by differential
expression in PBMCs of MMD and CCR2, transcripts
associated with monocyte activation and recruitment,
respectively. Our results indicate that high regular
bilberry intake may reduce endotoxemia and chronic
inflammation, the latter especially by directing the
immunity away from overactive innate cell mediated
responsiveness. Bilberry consumption may therefore
decrease the long term risk of cardiovascular and
metabolic diseases
KW - Bilberry
KW - endotoxemia
KW - gene expression
KW - inflammation
KW - metabolic syndrome
U2 - 10.17660/ActaHortic.2014.1017.45
DO - 10.17660/ActaHortic.2014.1017.45
M3 - Article
SN - 0567-7572
VL - 1017
SP - 369
EP - 380
JO - Acta Horticulturae
JF - Acta Horticulturae
IS - January
ER -