Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study

O.T. Mykkänen, P.V. Kirjavainen, L. Pulkkinen, H. Mykkänen, R. Törrönen, M. Kolehmainen, M. Adriaens, D.E. Laaksonen, Riitta Puupponen-Pimiä, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Obesity and its consequences on cardiometabolic health have been associated to low-grade inflammation. Anthocyanins are anti-inflammatory compounds in diet, and the major group of polyphenols found in wild European blueberries, bilberries (Vaccinium myrtillus). We have studied the effects of a bilberry-rich diet on inflammation and gene expression profile in peripheral blood mononuclear cells (PBMCs) in subjects with features of the metabolic syndrome. In a randomized, controlled dietary intervention study the bilberry group (n=15) consumed bilberries or berry products equivalent to 400 g of fresh berries daily for 8 weeks, while the participants in the control group (n=12) maintained their habitual diets. Inflammatory markers were measured before and after the intervention. The microarray profiling was done from 3 subjects in the bilberry group and further QPCR expression analyses from all subjects in both groups at both timepoints. From 50 differentially expressed transcripts for mRNA (P>0.005), five candidate genes; WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly selected for QPCR analyses. To further explore the interplay of dietary change and the affected pathways additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2), TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell receptor pathway) MMD, CCR2 (Monocyte and macrophage associated genes) were selected for QPCR analyses. Bilberries tended to decrease plasma concentrations of hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An inflammation score based on these markers and TNF-Alpha showed different trends between the bilberry and control groups. These changes were accompanied by differential expression in PBMCs of MMD and CCR2, transcripts associated with monocyte activation and recruitment, respectively. Our results indicate that high regular bilberry intake may reduce endotoxemia and chronic inflammation, the latter especially by directing the immunity away from overactive innate cell mediated responsiveness. Bilberry consumption may therefore decrease the long term risk of cardiovascular and metabolic diseases
Original languageEnglish
Pages (from-to)369-380
JournalActa Horticulturae
Volume1017
Issue numberJanuary
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

bilberries
Vaccinium myrtillus
endotoxemia
metabolic syndrome
mononuclear leukocytes
gene expression
inflammation
monocytes
small fruits
diet
genes
metabolic diseases
blueberries
endotoxins
interleukin-6
lipopolysaccharides
cardiovascular diseases
tumor necrosis factor-alpha
B-lymphocytes
anthocyanins

Keywords

  • Bilberry
  • endotoxemia
  • gene expression
  • inflammation
  • metabolic syndrome

Cite this

Mykkänen, O.T. ; Kirjavainen, P.V. ; Pulkkinen, L. ; Mykkänen, H. ; Törrönen, R. ; Kolehmainen, M. ; Adriaens, M. ; Laaksonen, D.E. ; Puupponen-Pimiä, Riitta ; Poutanen, Kaisa. / Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study. In: Acta Horticulturae. 2014 ; Vol. 1017, No. January. pp. 369-380.
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abstract = "Obesity and its consequences on cardiometabolic health have been associated to low-grade inflammation. Anthocyanins are anti-inflammatory compounds in diet, and the major group of polyphenols found in wild European blueberries, bilberries (Vaccinium myrtillus). We have studied the effects of a bilberry-rich diet on inflammation and gene expression profile in peripheral blood mononuclear cells (PBMCs) in subjects with features of the metabolic syndrome. In a randomized, controlled dietary intervention study the bilberry group (n=15) consumed bilberries or berry products equivalent to 400 g of fresh berries daily for 8 weeks, while the participants in the control group (n=12) maintained their habitual diets. Inflammatory markers were measured before and after the intervention. The microarray profiling was done from 3 subjects in the bilberry group and further QPCR expression analyses from all subjects in both groups at both timepoints. From 50 differentially expressed transcripts for mRNA (P>0.005), five candidate genes; WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly selected for QPCR analyses. To further explore the interplay of dietary change and the affected pathways additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2), TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell receptor pathway) MMD, CCR2 (Monocyte and macrophage associated genes) were selected for QPCR analyses. Bilberries tended to decrease plasma concentrations of hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An inflammation score based on these markers and TNF-Alpha showed different trends between the bilberry and control groups. These changes were accompanied by differential expression in PBMCs of MMD and CCR2, transcripts associated with monocyte activation and recruitment, respectively. Our results indicate that high regular bilberry intake may reduce endotoxemia and chronic inflammation, the latter especially by directing the immunity away from overactive innate cell mediated responsiveness. Bilberry consumption may therefore decrease the long term risk of cardiovascular and metabolic diseases",
keywords = "Bilberry, endotoxemia, gene expression, inflammation, metabolic syndrome",
author = "O.T. Mykk{\"a}nen and P.V. Kirjavainen and L. Pulkkinen and H. Mykk{\"a}nen and R. T{\"o}rr{\"o}nen and M. Kolehmainen and M. Adriaens and D.E. Laaksonen and Riitta Puupponen-Pimi{\"a} and Kaisa Poutanen",
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Mykkänen, OT, Kirjavainen, PV, Pulkkinen, L, Mykkänen, H, Törrönen, R, Kolehmainen, M, Adriaens, M, Laaksonen, DE, Puupponen-Pimiä, R & Poutanen, K 2014, 'Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study', Acta Horticulturae, vol. 1017, no. January, pp. 369-380. https://doi.org/10.17660/ActaHortic.2014.1017.45

Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study. / Mykkänen, O.T.; Kirjavainen, P.V.; Pulkkinen, L.; Mykkänen, H.; Törrönen, R.; Kolehmainen, M.; Adriaens, M.; Laaksonen, D.E.; Puupponen-Pimiä, Riitta; Poutanen, Kaisa.

In: Acta Horticulturae, Vol. 1017, No. January, 2014, p. 369-380.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Reduction of subacute endotoxemia could mediate beneficial effects of vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study

AU - Mykkänen, O.T.

AU - Kirjavainen, P.V.

AU - Pulkkinen, L.

AU - Mykkänen, H.

AU - Törrönen, R.

AU - Kolehmainen, M.

AU - Adriaens, M.

AU - Laaksonen, D.E.

AU - Puupponen-Pimiä, Riitta

AU - Poutanen, Kaisa

PY - 2014

Y1 - 2014

N2 - Obesity and its consequences on cardiometabolic health have been associated to low-grade inflammation. Anthocyanins are anti-inflammatory compounds in diet, and the major group of polyphenols found in wild European blueberries, bilberries (Vaccinium myrtillus). We have studied the effects of a bilberry-rich diet on inflammation and gene expression profile in peripheral blood mononuclear cells (PBMCs) in subjects with features of the metabolic syndrome. In a randomized, controlled dietary intervention study the bilberry group (n=15) consumed bilberries or berry products equivalent to 400 g of fresh berries daily for 8 weeks, while the participants in the control group (n=12) maintained their habitual diets. Inflammatory markers were measured before and after the intervention. The microarray profiling was done from 3 subjects in the bilberry group and further QPCR expression analyses from all subjects in both groups at both timepoints. From 50 differentially expressed transcripts for mRNA (P>0.005), five candidate genes; WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly selected for QPCR analyses. To further explore the interplay of dietary change and the affected pathways additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2), TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell receptor pathway) MMD, CCR2 (Monocyte and macrophage associated genes) were selected for QPCR analyses. Bilberries tended to decrease plasma concentrations of hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An inflammation score based on these markers and TNF-Alpha showed different trends between the bilberry and control groups. These changes were accompanied by differential expression in PBMCs of MMD and CCR2, transcripts associated with monocyte activation and recruitment, respectively. Our results indicate that high regular bilberry intake may reduce endotoxemia and chronic inflammation, the latter especially by directing the immunity away from overactive innate cell mediated responsiveness. Bilberry consumption may therefore decrease the long term risk of cardiovascular and metabolic diseases

AB - Obesity and its consequences on cardiometabolic health have been associated to low-grade inflammation. Anthocyanins are anti-inflammatory compounds in diet, and the major group of polyphenols found in wild European blueberries, bilberries (Vaccinium myrtillus). We have studied the effects of a bilberry-rich diet on inflammation and gene expression profile in peripheral blood mononuclear cells (PBMCs) in subjects with features of the metabolic syndrome. In a randomized, controlled dietary intervention study the bilberry group (n=15) consumed bilberries or berry products equivalent to 400 g of fresh berries daily for 8 weeks, while the participants in the control group (n=12) maintained their habitual diets. Inflammatory markers were measured before and after the intervention. The microarray profiling was done from 3 subjects in the bilberry group and further QPCR expression analyses from all subjects in both groups at both timepoints. From 50 differentially expressed transcripts for mRNA (P>0.005), five candidate genes; WDSUB1, COX7B, RGS18, DAPP1 and TICAM1, were randomly selected for QPCR analyses. To further explore the interplay of dietary change and the affected pathways additional genes of LBP, TNFRSF12A, RIPK-1, Ly96 (MD2), TAB-2 (Toll-like receptor pathway); CD19, CD72 (B-cell receptor pathway) MMD, CCR2 (Monocyte and macrophage associated genes) were selected for QPCR analyses. Bilberries tended to decrease plasma concentrations of hsCRP, IL-6 and LPS (lipopolysaccharide, endotoxin). An inflammation score based on these markers and TNF-Alpha showed different trends between the bilberry and control groups. These changes were accompanied by differential expression in PBMCs of MMD and CCR2, transcripts associated with monocyte activation and recruitment, respectively. Our results indicate that high regular bilberry intake may reduce endotoxemia and chronic inflammation, the latter especially by directing the immunity away from overactive innate cell mediated responsiveness. Bilberry consumption may therefore decrease the long term risk of cardiovascular and metabolic diseases

KW - Bilberry

KW - endotoxemia

KW - gene expression

KW - inflammation

KW - metabolic syndrome

U2 - 10.17660/ActaHortic.2014.1017.45

DO - 10.17660/ActaHortic.2014.1017.45

M3 - Article

VL - 1017

SP - 369

EP - 380

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

IS - January

ER -