Reed canary grass as a feedstock for 2nd generation bioethanol production

Anne Kallioinen (Corresponding Author), Jaana Uusitalo, K. Pahkala, M. Kontturi, Liisa Viikari, Niklas von Weymarn, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

The enzymatic hydrolysis and fermentation of reed canary grass, harvested in the spring or autumn, and barley straw were studied. Steam pretreated materials were efficiently hydrolysed by commercial enzymes with a dosage of 10–20 FPU/g d.m. Reed canary grass harvested in the spring was hydrolysed more efficiently than the autumn-harvested reed canary grass. Additional β-glucosidase improved the release of glucose and xylose during the hydrolysis reaction. The hydrolysis rate and level of reed canary grass with a commercial Trichoderma reesei cellulase could be improved by supplementation of purified enzymes. The addition of CBH II improved the hydrolysis level by 10% in 48 hours’ hydrolysis. Efficient mixing was shown to be important for hydrolysis already at 10% dry matter consistency. The highest ethanol concentration (20 g/l) and yield (82%) was obtained with reed canary grass at 10% d.m. consistency.
Original languageEnglish
Pages (from-to)669-672
Number of pages3
JournalBioresource Technology
Volume123
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Bioethanol
Feedstocks
hydrolysis
Hydrolysis
grass
Enzymes
Glucosidases
autumn
Xylose
Enzymatic hydrolysis
Cellulase
enzyme
Steam
Straw
Fermentation
Glucose
barley
Ethanol
straw
fermentation

Keywords

  • Barley straw
  • enzymatic hydrolysis
  • pretreatment
  • reed canary grass
  • simultaneous saccharification and fermentation

Cite this

Kallioinen, Anne ; Uusitalo, Jaana ; Pahkala, K. ; Kontturi, M. ; Viikari, Liisa ; von Weymarn, Niklas ; Siika-aho, Matti. / Reed canary grass as a feedstock for 2nd generation bioethanol production. In: Bioresource Technology. 2012 ; Vol. 123. pp. 669-672.
@article{d6a49d55f22f486cb811ff2f1243410a,
title = "Reed canary grass as a feedstock for 2nd generation bioethanol production",
abstract = "The enzymatic hydrolysis and fermentation of reed canary grass, harvested in the spring or autumn, and barley straw were studied. Steam pretreated materials were efficiently hydrolysed by commercial enzymes with a dosage of 10–20 FPU/g d.m. Reed canary grass harvested in the spring was hydrolysed more efficiently than the autumn-harvested reed canary grass. Additional β-glucosidase improved the release of glucose and xylose during the hydrolysis reaction. The hydrolysis rate and level of reed canary grass with a commercial Trichoderma reesei cellulase could be improved by supplementation of purified enzymes. The addition of CBH II improved the hydrolysis level by 10{\%} in 48 hours’ hydrolysis. Efficient mixing was shown to be important for hydrolysis already at 10{\%} dry matter consistency. The highest ethanol concentration (20 g/l) and yield (82{\%}) was obtained with reed canary grass at 10{\%} d.m. consistency.",
keywords = "Barley straw, enzymatic hydrolysis, pretreatment, reed canary grass, simultaneous saccharification and fermentation",
author = "Anne Kallioinen and Jaana Uusitalo and K. Pahkala and M. Kontturi and Liisa Viikari and {von Weymarn}, Niklas and Matti Siika-aho",
year = "2012",
doi = "10.1016/j.biortech.2012.07.023",
language = "English",
volume = "123",
pages = "669--672",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Reed canary grass as a feedstock for 2nd generation bioethanol production. / Kallioinen, Anne (Corresponding Author); Uusitalo, Jaana; Pahkala, K.; Kontturi, M.; Viikari, Liisa; von Weymarn, Niklas; Siika-aho, Matti.

In: Bioresource Technology, Vol. 123, 2012, p. 669-672.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Reed canary grass as a feedstock for 2nd generation bioethanol production

AU - Kallioinen, Anne

AU - Uusitalo, Jaana

AU - Pahkala, K.

AU - Kontturi, M.

AU - Viikari, Liisa

AU - von Weymarn, Niklas

AU - Siika-aho, Matti

PY - 2012

Y1 - 2012

N2 - The enzymatic hydrolysis and fermentation of reed canary grass, harvested in the spring or autumn, and barley straw were studied. Steam pretreated materials were efficiently hydrolysed by commercial enzymes with a dosage of 10–20 FPU/g d.m. Reed canary grass harvested in the spring was hydrolysed more efficiently than the autumn-harvested reed canary grass. Additional β-glucosidase improved the release of glucose and xylose during the hydrolysis reaction. The hydrolysis rate and level of reed canary grass with a commercial Trichoderma reesei cellulase could be improved by supplementation of purified enzymes. The addition of CBH II improved the hydrolysis level by 10% in 48 hours’ hydrolysis. Efficient mixing was shown to be important for hydrolysis already at 10% dry matter consistency. The highest ethanol concentration (20 g/l) and yield (82%) was obtained with reed canary grass at 10% d.m. consistency.

AB - The enzymatic hydrolysis and fermentation of reed canary grass, harvested in the spring or autumn, and barley straw were studied. Steam pretreated materials were efficiently hydrolysed by commercial enzymes with a dosage of 10–20 FPU/g d.m. Reed canary grass harvested in the spring was hydrolysed more efficiently than the autumn-harvested reed canary grass. Additional β-glucosidase improved the release of glucose and xylose during the hydrolysis reaction. The hydrolysis rate and level of reed canary grass with a commercial Trichoderma reesei cellulase could be improved by supplementation of purified enzymes. The addition of CBH II improved the hydrolysis level by 10% in 48 hours’ hydrolysis. Efficient mixing was shown to be important for hydrolysis already at 10% dry matter consistency. The highest ethanol concentration (20 g/l) and yield (82%) was obtained with reed canary grass at 10% d.m. consistency.

KW - Barley straw

KW - enzymatic hydrolysis

KW - pretreatment

KW - reed canary grass

KW - simultaneous saccharification and fermentation

U2 - 10.1016/j.biortech.2012.07.023

DO - 10.1016/j.biortech.2012.07.023

M3 - Article

VL - 123

SP - 669

EP - 672

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -